70014-M

T7Select^® Packaging Kit

Ultimate efficiency in T7Select library construction

• Synonym(s): T7Select^® Phage Display System, T7Select^® System
• UNSPSC Code: 41106617
• NACRES: NA.54

Properties

• Quality Level: 100
• manufacturer/tradename: Novagen^®
• storage condition: OK to freeze; avoid repeated freeze/thaw cycles
• shipped in: dry ice
• storage temp.: −70°C

Description

General description

Novagen′s T7Select^® Phage Display System is a novel phage display system that takes advantage of the properties of bacteriophage T7. This system is easy to use and has the capacity to display peptides up to about 50 amino acids in size in high copy number (415 per phage), and peptides or proteins up to about 1200 amino acids in low copy number (0.1-1 per phage) or mid-copy number (5-15 per phage). Phage assembly takes place in the E. coli cytoplasm and mature phage are released by cell lysis. Unlike the filamentous systems, peptides or proteins displayed on the surface of T7 do not need to be capable of secretion through the cell membrane, a necessary step in filamentous phage assembly.

T7Select Packaging Extracts are optimized for maximum packaging efficiencies of T7Select vectors ligated with insert. The high efficiency extracts (> 2 x 10⁸ pfu/µg with T7Select Packaging Control DNA) make it easy to produce a library containing at least 5 x 10⁷ recombinants per microgram of arms. The extracts are made from a specially engineered phage that reduces the nonrecombinant cloning background to below 0.1%.

Components

• 6- T7 Packaging Extracts
• 1 µg- T7Select Packaging Control DNA
• 0.2 ml- BL21 glycerol stock
• 0.2 ml- BLT5403 glycerol stock
• 0.2 ml- BLT5615 glycerol stock

Warning

Toxicity: Standard Handling

Legal Information

NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany

T7SELECT is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges MilliporeSigma to request certain information about you and the establishment where the GMOs are being handled. Clickhere for Enduser Declaration (EUD) Form

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 3