70018-M
T7Select® Biopanning Kit
Matched reagents for selecting binders from T7Select libraries
About This Item
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Quality Level
manufacturer/tradename
Novagen®
storage condition
OK to freeze
avoid repeated freeze/thaw cycles
Related Categories
General description
Biopanning selection
Biopanning requires both binding and elution conditions that maintain the infectivity of the phage. The stability of the T7 phage particle enables the use of a variety of elution conditions during biopanning. Phage maintain infectivity following treatment with 1% SDS, 5 M NaCl, 4 M urea, 2 M guanidine-HCl, 10 mM EDTA, reducing conditions (up to 100 mM DTT), and a pH range of 4 to 10. The wide range of conditions available for T7Select biopanning expands the range of targets that can be used successfully.
Components
- 25 g Blocking Reagent
- 2 x 50 ml 10X TBST Wash Buffer
- 10 ml T7 Elution Buffer
- 0.2 ml BL21 Glycerol Stock
- 0.2 ml BLT5403 Glycerol Stock
- 0.2 ml BLT5615 Glycerol Stock
- 1010 pfu T7Select Positive Control Lysate
- 1010 pfu T7Select Negative Control Lysate
- 50 μl S-protein HRP Conjugate
- 2 ml T7Select S-protein
- 50 ml SuperSignal Substrate
- pkg/25 g LOCATOR Luminescent Labels
- pkg/25 Development Folders
Warning
Legal Information
Storage Class Code
10 - Combustible liquids
WGK
WGK 3
Certificates of Analysis (COA)
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