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Assay
≥95% (SDS-PAGE)
Quality Level
form
lyophilized
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze
impurities
≤10% S100 α-chain (Western blot)
solubility
50 mM Tris buffer, pH 8.0: 5 mg/mL
shipped in
ambient
storage temp.
−20°C
General description
Native homodimeric S-100b isoform composed of ββ-subunits, purified from bovine brain. The S100 calcium-binding protein B (S100B) exists in the form of homo- or hetero-dimers comprised of two subunits, A and B. Specifically, the S-100AB and S-100BB types are referred to as S100B protein and are known for their strong association with nervous tissue. This protein can be found in the cytosol of glial and Schwann cells. [1] The gene responsible for encoding the S100B protein is situated in the chromosomal region 21q22. [2] Under nonreducing conditions both the dimeric and monomeric forms may be visible.
Application
- to study the interaction between the serotonin 5-HT7 receptor and the calcium-binding protein S100B using surface plasmon resonance-based method. [3]
- as a probe to detect and study the binding interactions with proteins during the far-western blotting technique. [4]
- to investigate the role of S100B in relation to blood-brain barrier (BBB) integrity and its potential involvement in the production of autoantibodies against S100B. [5]
- to study the mechanism of communication between thermogenic adipocytes and sympathetic neurons mediated by Calsyntenin-3β and S100b, providing insights into the regulation of sympathetic innervation in adipose tissue. [6]
Biochem/physiol Actions
Warning
Physical form
Reconstitution
Other Notes
Baudier, J., et al. 1992. Proc. Natl. Acad. Sci. USA 89, 11627.
Donato, R. 1991. Cell Calcium12, 713.
Isobe, T., et al. 1977. Biochim. Biophys. Acta494, 222.
Legal Information
Storage Class Code
11 - Combustible Solids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
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