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5.06026

Sigma-Aldrich

X5050

≥97% (HPLC), powder, REST inhibitor, Calbiochem®

Synonym(s):

REST Inhibitor, X5050, Neuron Resctrictive Silencer Factor (NRSF) Inhibitor, X5050, X-5050, Repressor Element-1 Silencing Transcription Factor (REST) Inhibitor, X5050

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About This Item

Empirical Formula (Hill Notation):
C17H15N3O3
Molecular Weight:
309.32
UNSPSC Code:
12352200
NACRES:
NA.77
Pricing and availability is not currently available.

Product Name

REST Inhibitor, X5050,

Assay

≥97% (HPLC)

Quality Level

100

form

powder

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze
protect from light

color

light beige

solubility

DMSO: 100 mg/mL

storage temp.

2-8°C

General description

A cell-permeable benzoimidazole derivative that acts as an inhibitor of repressor element-1 silencing transcription factor (REST, a Kruppel-type zinc finger transcription factor). Induces a dose-dependent reduction in the 122 kDa isoforms of REST in HEK cells. It is believed to target REST degradation, but not its expression or binding to repressor element binding sequence. Shown to rescue REST-regulated gene expressions, such as BDNF in the prefrontal cortex in mice with quinolinic acid-induced striatal lesions. Acute ventricular delivery of X5050 increases the expression of BDNFand other REST-regulated genes. Also shown to promote the expression of BDNF and SNAP25 in human neural stem cells via degradation of REST.

Please note that the molecular weight for this compound is batch-specific due to variable water content.

Biochem/physiol Actions

Cell permeable: yes
Primary Target
REST

Packaging

Packaged under inert gas

Warning

Toxicity: Standard Handling (A)

Reconstitution

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.

Other Notes

Charbord, J., et al, 2013, Stem Cells31, 1816.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Questions

  1. What is the half life of this compound in media/water/PBS/etc.?

    1 answer

    1. The half-life of this inhibitor has not been determined. A brief search of the literature revealed a publication that may be helpful. Please see the link below to review this publication:
      'High throughput screening for inhibitors of REST in neural derivatives of human embryonic stem cells reveals a chemical compound that promotes expression of neuronal genes'
      https://oup.silverchair-cdn.com/oup/backfile/Content_public/Journal/stmcls/31/9/10.1002_stem.1430/2/stmcls_31_9_1816.pdf?Expires=1706884944&Signature=xAKu8eDY7vVgmAEUYmzNOxHlm5Hzqfw-oY3~FAQqAPeQs3kcLCLN5hin0vKHkkX7HhANhhF-n1HvYJq1WJf-T-jrYoE0iJ6Sj6sLZVtQ-sJb3qeG~~7kgQZzW~gC5zs9WLy3n-79swOK02yRBZYRMPmN5RMiY6fuTfA9YbKjCHEPaiiKQ7dRmyUktu7bp98fHlW0i6-ZyzOJQKN8iXdaSIprT0bAr5b3r7BFquRU9doPYNVwDbspKjykXyB~kdM7oIvygrSDJ9454GIstiVK4Bp8I5jk0LkYnb0Qu4AtNnATerrQAh-MiPRY2ESIqEgd1xtgRFmOL0Fs7BFY3vUSow__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA

      Helpful?

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