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219475

CENTA β-Lactamase Substrate

≥98% (TLC), powder, β-lactamase substrate, Calbiochem®

Synonym(s):

CENTA β-Lactamase Substrate

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About This Item

CAS Number:
80072-86-2
UNSPSC Code:
12352200
NACRES:
NA.54
Assay:
≥98% (TLC)
Form:
powder
Storage condition:
OK to freeze
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Product Name

CENTA β-Lactamase Substrate, CENTA β-Lactamase Substrate is a chromogenic β-lactamase substrate for both Gram-negative and Gram-positive bacteria.

Quality Segment

description

RTECS - OD2265000

assay

≥98% (TLC)

form

powder

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze

color

yellow

solubility

25 mM phosphate buffer, pH 7.0: 2.5 mg/mL, methanol: 50 mg/mL

shipped in

ambient

storage temp.

−20°C

General description

Chromogenic β-lactamase substrate for both gram-negative and gram-positive bacteria that is comparable to other chromogens (PADAC Substrate and nitrocefin). Hydrolysis of the beta-lactam ring causes a color change from light yellow (λmax: 340 nm) to chrome yellow (λmax: 405 nm). Relatively unaffected by commonly used microbiological media and human serum. Stable in H2O and in the presence of cysteine, mercaptoethanol and dimercaptopropanol.

Please note that the molecular weight for this compound is batch-specific due to variable water content.

Preparation Note

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.

Other Notes

Jones, R.N., et al. 1982. J. Clin. Microbiol.15, 954.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Standard Handling (A)

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This Item
484400203783487500
CENTA β-Lactamase Substrate CENTA β-Lactamase Substrate is a chromogenic β-lactamase substrate for both Gram-negative and Gram-positive bacteria.

Sigma-Aldrich

219475

CENTA β-Lactamase Substrate

Nitrocefin A chromogenic β-lactamase substrate that undergoes distinctive color change from yellow as the amide bond in the β-lactam ring is hydrolyzed by β-lactamase.

Sigma-Aldrich

484400

Nitrocefin

5-Bromo-4-chloro-3-indolyl-β-D-glucuronic Acid, Cyclohexylammonium Salt Substrate for β-glucuronidase that produces insoluble, intense indigo-blue chromophore (615 nm) after enzymatic hydrolysis.

Sigma-Aldrich

203783

5-Bromo-4-chloro-3-indolyl-β-D-glucuronic Acid, Cyclohexylammonium Salt

p-Nitrophenyl-β-D-glucuronide Chromogenic substrate for β-glucuronidase (Km = ~0.22 mM).

Sigma-Aldrich

487500

p-Nitrophenyl-β-D-glucuronide

description

CENTA β-Lactamase Substrate is a chromogenic β-lactamase substrate for both Gram-negative and Gram-positive bacteria.

description

A chromogenic β-lactamase substrate that undergoes distinctive color change from yellow as the amide bond in the β-lactam ring is hydrolyzed by β-lactamase.

description

Substrate for β-glucuronidase that produces insoluble, intense indigo-blue chromophore (615 nm) after enzymatic hydrolysis.

description

Chromogenic substrate for β-glucuronidase (Km = ~0.22 mM).

assay

≥98% (TLC)

assay

≥95% (UV)

assay

≥98% (HPLC)

assay

≥98% (HPLC)

form

powder

form

solid

form

solid

form

solid

manufacturer/tradename

Calbiochem®

manufacturer/tradename

Calbiochem®

manufacturer/tradename

Calbiochem®

manufacturer/tradename

Calbiochem®

Quality Level

100

Quality Level

200

Quality Level

100

Quality Level

100

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

2-8°C

solubility

methanol: 50 mg/mL, 25 mM phosphate buffer, pH 7.0: 2.5 mg/mL

solubility

soluble (Preparing a Nitrocefin (500 µg/ml) Solution
• Dissolve 1 mg Nitrocefin in 100 µl dimethylsulfoxide (DMSO) and vortex.
• Add 1.9 ml phosphate buffer (100 mM, pH 7) to produce 2 ml total volume.
• This yields a working Nitrocefin solution of 500 µg/ml (~1 mM), which is suitable for most applications.
• Nitrocefin, particularly in solution, is very sensitive to light.)

solubility

soluble (2% in DMF)

solubility

water: 10 mg/mL

Storage Class

11 - Combustible Solids

WGK

WGK 3

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

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