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12-309

Sigma-Aldrich

HeLa Nuclear Extract

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About This Item

UNSPSC Code:
41105330
eCl@ss:
32011807
NACRES:
NA.41
Pricing and availability is not currently available.

biological source

human

Quality Level

100

form

liquid

manufacturer/tradename

Upstate®

concentration

2.0 mg/mL

technique(s)

western blot: suitable

shipped in

dry ice

General description

Nuclear extract prepared from human HeLa cells using a modified protocol of Dignam, et al., which contains a variety of DNA binding proteins and transcription factors.

Cell Line Description

HeLa

Quality

Routinely evaluated as a positive control for many of Upstates antibodies.

Physical form

RIPA sample buffer containing inhibitors. Frozen solution.

Storage and Stability

Stable for 6 months at -70°C from date of shipment.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Pictograms

Exclamation mark
GHS07

Signal Word

Warning

Hazard Statements

H319

Hazard Classifications

Eye Irrit. 2

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Tabetha Sundin et al.
Biochimie, 94(12), 2639-2648 (2012-08-21)
Isoprenoids are recognized for their ability to suppress carcinogenic processes in vivo and in vitro. We previously established that the isoprenoid, perillyl alcohol, acted mechanistically on translation of specific proteins through modulation of mechanistic target of rapamycin (mTOR) signaling. Telomerase-the
J D Dignam et al.
Nucleic acids research, 11(5), 1475-1489 (1983-03-11)
We have developed a procedure for preparing extracts from nuclei of human tissue culture cells that directs accurate transcription initiation in vitro from class II promoters. Conditions of extraction and assay have been optimized for maximum activity using the major

Questions

1–2 of 2 Questions  

  1. Is this product in vitro transcription grade? What are the components provided with this item? Can I used this item to test for human rna polymerase 2 transcription?

    1 answer

    1. This product is not tested for a specific application, such as transcription efficiency. The material is supplied as a single vial of a 50 ug/25 uL solution containing RIPA buffer and inhibitors. No other components are included. Note that unless otherwise stated this product is intended for research use only and is not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

      Please see the link below to review available Certificates of Analysis:
      https://www.emdmillipore.com/product/HeLa-Nuclear-Extract,MM_NF-12-309#anchor_COA

      Helpful?

  2. Can I use this HeLa Nuclear Extract for deadenylase assay? specifically, Does this product contain the complex of deadenylase in addition to a variety of DNA binding proteins and transcription factors?

    1 answer

    1. This product has not been tested for use in deadenylation. There is no reason to suspect that it would not be suitable. However, the product is stored in RIPA buffer. Please check the literature for potential interference of RIPA in the adenylase assay reaction.

      Helpful?

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