1.04170

Erythrosine B (C.I. 45430)

• Empirical Formula (Hill Notation): C₂₀H₆I₄Na₂O₅
• CAS Number: 16423-68-0
• Molecular Weight: 879.86
• UNSPSC Code: 41116130
• NACRES: NA.21
• MDL number: MFCD00144257

Properties

• form: solid
• Quality Segment: 200
• potency: 1840 mg/kg LD₅₀, oral (Rat)
• composition: Dye, ≥80% gravimetric
• pH: 6-7 (20 °C, 10 g/L in H₂O)
• solubility: 100 g/L
• bulk density: 470 kg/m³
• application(s): diagnostic assay manufacturing; hematology; histology
• storage temp.: 2-30°C
• SMILES string: [Na+].[Na+].Ic1c2c(cc(c1[O-])I)C(=C4C(=C(C(=O)C(=C4)I)I)O2)c3c(cccc3)C(=O)[O-]
• InChI: 1S/C20H8I4O5.2Na/c21-11-5-9-13(7-3-1-2-4-8(7)20(27)28)10-6-12(22)17(26)15(24)19(10)29-18(9)14(23)16(11)25;;/h1-6,25H,(H,27,28);;/q;2*+1/p-2
• InChI key: IINNWAYUJNWZRM-UHFFFAOYSA-L

Description

General description

Erythrosin B (C.I. 45430)for microscopy Certistain^®, is a dry dye for microscopy that is often used as a counterstain, vizualising proteins, connective tissues, fibers and keratin in sample material of human origin. Depending on user requirements and personal preferences, it can be used for the preparation of the commonly used aqueous or alcoholic Erythrosin B staining solutions. As the dye belongs to the portfolio of Certistain^® dyes, it is chemically analyzed according to strict specifications before release and a high staining quality, with reliable staining results is guaranteed.

Application

for microscopy Certistain^®

Analysis Note

Dye content (gravimetrically): ≥ 80 %
Identity
- sodium:
passes test
- UV/VIS-Spectrum: passes test
Absorption maximum λ max (water): 524 - 527 nm
Spec. Absorptivity A 1%/1cm (λ_max; 0.005 g/l; water): 930 - 1170
TLC-Test: passes test
Loss on drying (110 °C): ≤ 10 %
Suitability for microscopy: passes test

Legal Information

CERTISTAIN is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• pictograms: GHS07,GHS09
• signalword: Warning
• hcodes: H302 - H411
• pcodes: P264 - P270 - P273 - P301 + P312 - P391 - P501
• Hazard Classifications: Acute Tox. 4 Oral - Aquatic Chronic 2
• Storage Class: 11 - Combustible Solids
• wgk: WGK 1
• flash_point_f: 359.6 °F - closed cup
• flash_point_c: 182 °C - closed cup