H9041

Vascular Endothelial Growth Factor 121 human

HumanKine^®, ≥95% (SDS-PAGE), recombinant, expressed in HEK 293 cells, lyophilized powder, suitable for cell culture

• MDL number: MFCD00286542
• UNSPSC Code: 12352202
• NACRES: NA.77

Properties

• product name: Vascular Endothelial Growth Factor 121 human, VEGF121, recombinant, expressed in HEK 293 cells, HumanKine^®, suitable for cell culture
• biological source: human
• Quality Level: 100
• recombinant: expressed in HEK 293 cells
• Assay: ≥95% (SDS-PAGE)
• form: lyophilized powder
• potency: ≤15 ng/mL EC₅₀
• quality: endotoxin tested
• mol wt: dimer 37 kDa (glycosylated); trimer 50 kDa (glycosylated)
• packaging: pkg of 10 μg
• manufacturer/tradename: HumanZyme
• storage condition: avoid repeated freeze/thaw cycles
• technique(s): cell culture | mammalian: suitable
• impurities: ≤1 EU/μg
• UniProt accession no.: P15692
• storage temp.: −20°C
• Gene Information: human ... VEGFA(7422)

Description

General description

HumanPro VEGF-121(vascular endothelial growth factor 121) is an isoform of VEGFA and is expressed in human HEK 293 cells as a disulfide linked, glycosylated cytokine with an apparent molecular mass of 37 kDa as a homodimer and 50 kDa as a homotrimer. VEGF-A exists in three different isoforms VEGF-121, VEGF165 is an angiogenic properties. Production in human HEK 293 cells offers authentic glycosylation. Glycosylation contributes to stability in cell growth media and other applications.
There are five types of VEGF ligands identified in mammals, which exists as different splice variants.VEGFA is a dimeric glycoprotein consisting two monomers antiparallel to each other.

Biochem/physiol Actions

Vascular endothelial growth factor 121 (VEGF121) belongs to the PDGF/VEGF growth factor family. Many cell types secrete VEGF, and it is a potent angiogenic factor and mitogen that stimulates proliferation, migration, and formation of endothelial cells. VEGF stimulates permeabilization of blood vessels and is present in some tumors of the nervous system. VEGF is induced by hypoxia, oncogene mutations, and cytokines such as IL-1, IL-8, and TNF-α.

Physical form

Lyophilized from a 0.2 μm filtered solution of 1X PBS

Analysis Note

The specific activity was determined by the dose-dependent stimulation of the proliferation of HUVEC cells (human umbilical vein endothelial cells).

Legal Information

HumanKine is a registered trademark of Proteintech Group, Inc. and Humanzyme, Inc

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable