F3133
Fibroblast Growth Factor from bovine pituitary
suitable for cell culture
Synonym(s):
FGF
Sign Into View Organizational & Contract Pricing
All Photos(1)
About This Item
Recommended Products
biological source
bovine pituitary glands
Quality Level
form
lyophilized powder
potency
1-100 ng/mL
mol wt
16.4 kDa
packaging
pkg of 10 and 5X10 μg
storage condition
avoid repeated freeze/thaw cycles
technique(s)
cell culture | mammalian: suitable
impurities
endotoxin, tested
color
white to off-white
storage temp.
−20°C
Looking for similar products? Visit Product Comparison Guide
General description
Fibroblast growth factors (FGF) are glycoproteins concealed in the extracellular matrix and cell surface. There are around 22 different types of FGFs known. These factors weigh in the range of 17 to 34kDa and they possess a conserved sequence of 120 amino acids.
Application
A natural mitogenic growth factor used in cell culture applications to study cell signaling and to promote fibroblast and endothelial cell growth and survival.
Fibroblast Growth Factor from bovine pituitary has been used in in vitro testicular cell culture. It has also been used as a component of myogenic culture medium.
Biochem/physiol Actions
Fibroblast growth factors (FGF) actively participate in cellular responses including embryogenesis and organogenesis. It specifically controls cell proliferation, differentiation and migration. FGF is known to regulate nervous system functions, tissue repair mechanism and wound healing. It also controls angiogenesis of tumor cells. Abnormal FGF mediated signaling induces tumor angiogenesis, by stimulating cancer cell proliferation and survival. FGFs carries out its function by mediating through four receptor tyrosine kinases : FGF-receptors FGFR1, FGFR2, FGFR3 and FGFR4.
Quality
Although the major mitogenic component of this product is FGF2, the activity per unit weight of protein is less than that of purified FGF2.
Physical form
Lyophilized from a 0.2 μm filtered solution in phosphate buffered saline containing 0.1 mg/ml BSA.
Preparation Note
Purified glands by a modification of the method of Gospodarowicz.
Analysis Note
The biological activity is measured using a 3-day MTT assay.
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Customers Also Viewed
Modulation of osteogenic, adipogenic and myogenic differentiation of mesenchymal stem cells by submicron grooved topography
Wang PY, et al.
Journal of Materials Science. Materials in Medicine, 23(12), 3015-3028 (2012)
Characterization and In Vitro Differentiation of Korean Ring-Necked Pheasant (Phasianus colchicus) Male Germ Cells
Jeong DK, et al.
Journal of Embryology transfer , 29(4), 351-359 (2014)
Cellular signaling by fibroblast growth factor receptors
Eswarakumar VP, et al.
Cytokine & Growth Factor Reviews, 16(2), 139-149 (2005)
Jin Gyoung Jung et al.
Biology of reproduction, 76(1), 173-182 (2006-10-13)
We recently succeeded in inducing germline transmission by transferring chicken testicular cells into heterologous testes. This study was designed subsequently to identify pluripotent cells in the testicular cells, which would induce the germline transmission. Testicular cells retrieved from juvenile (4-wk-old)
Rocky S Tuan et al.
The Journal of the American Academy of Orthopaedic Surgeons, 21(5), 303-311 (2013-05-03)
Cartilage damaged by trauma has a limited capacity to regenerate. Current methods of managing small chondral defects include palliative treatment with arthroscopic débridement and lavage, reparative treatment with marrow-stimulation techniques (eg, microfracture), and restorative treatment, including osteochondral grafting and autologous
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service