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Merck

HPLC Analysis of Proteins on Proteomixยฎ SCX Columns, Effect of Particle Size of Resolution

HPLC Analysis of Proteins on Proteomix® SCX Columns, Effect of Particle Size of Resolution application for HPLC

Materials

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Z777135

Proteomixยฎ SCX-NP10 Strong Cation Exchange Column

L ร— I.D. 5 cm ร— 4.6 mm, 10 μm particle size, stainless steel

Z777146

Proteomixยฎ SCX-NP1.7 Strong Cation Exchange Column

L ร— I.D. 5 cm ร— 4.6 mm, 1.7 μm particle size, stainless steel

Z777151

Proteomixยฎ SCX-NP3 Strong Cation Exchange Column

L ร— I.D. 5 cm ร— 4.6 mm, 3 μm particle size, stainless steel

Z777159

Proteomixยฎ SCX-NP5 Strong Cation Exchange Column

L ร— I.D. 5 cm ร— 4.6 mm, 5 μm particle size, stainless steel

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L6876

Lysozyme from chicken egg white

powder or granules, ≥39,000 units/mg protein

C2506

Cytochrome c from equine heart

≥95% (SDS-PAGE)

R6513

Ribonuclease A from bovine pancreas

for molecular biology, ≥70 Kunitz units/mg protein, lyophilized

CONDITIONS

column

Proteomix SCX-NP1.7, 5 cm x 4.6 mm I.D., 1.7 μm particles (Z777146)

column

Proteomix SCX-NP3, 5 cm x 4.6 mm I.D., 3 μm particles (Z777151)

mobile phase

(A) 20 mM sodium phosphate buffer, pH 6.0, (B) A + 1.0 M NaCl

gradient

0-25 min from 0-75% B, 15 min prewash

flow rate

0.75 mL/min

column temp.

Ambient

detector

214 nm

injection

5 μL

sample

each 1 mg/mL

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Proteomix is a registered trademark of Sepax Technologies

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Related Product Categories

HPLC ColumnsGC Columns & AccessoriesTLC Plates & AdsorbentsSolid Phase Microextraction (SPME)Solid Phase Extraction & QuEChERS

Related Applications

Small Molecule HPLCLarge Molecule HPLCGas Chromatography (GC)TLCSolid Phase Microextraction (SPME)Solid Phase Extraction (SPE)QuEChERS