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HPLC Analysis of Protein Standards on Zenix® SEC-300 using Different Buffer Systems

HPLC Analysis of Protein Standards on Zenix® SEC-300 using Different Buffer Systems application for HPLC

Materials

분석 컬럼

제품 번호
설명
가격

Zenix® SEC-300 Gel Filtration Column, 3 μm

L × I.D. 30 cm × 4.6 mm, 3 μm particle size, stainless steel

이동상 성분

제품 번호
설명
가격

Ammonium bicarbonate

BioUltra, ≥99.5% (T)

표준

제품 번호
설명
가격

Myoglobin from equine heart

≥90% (SDS-PAGE), essentially salt-free, lyophilized powder

Uracil

≥99.0%

Thyroglobulin from bovine thyroid

(For Gel Filtration Chromatography)

IgG from rabbit serum

reagent grade, ≥95% (SDS-PAGE), essentially salt-free, lyophilized powder

Bovine Serum Albumin

lyophilized powder, ≥96% (agarose gel electrophoresis)

CONDITIONS

column

Zenix SEC-300, 30 cm x 4.6 mm I.D., 3 μm particles (Z777028)

mobile phase

[A] 0.15 M potassium phosphate monobasic, pH 7.0 with phosphoric acid; [B] 0.1 M ammonium bicarbonate (pH unadjusted)

flow rate

0.3 mL/min

column temp.

25 °C

detector

UV, 280 nm

detector

1000 psi

injection

2.5 μL

sample

in mobile phase

설명

분석 메모

This application compares selectivity of proteins when chromotographed by size exclusion with different buffer systems.

법적 정보

Zenix is a registered trademark of Sepax Technologies