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Merck

HPLC Analysis of Peptides and Proteins on Proteomixยฎ SCX-NP1.7, 5 cm x 4.6 mm I.D. Column

HPLC Analysis of Peptides and Proteins on Proteomix® SCX-NP1.7, 5 cm x 4.6 mm I.D. Column application for HPLC

Materials

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Proteomixยฎ SCX-NP1.7 Strong Cation Exchange Column

L ร— I.D. 5 cm ร— 4.6 mm, 1.7 μm particle size, stainless steel

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Lysozyme from chicken egg white

powder or granules, ≥39,000 units/mg protein

Ribonuclease A from bovine pancreas

Type I-A, powder, ≥60% RNase A basis (SDS-PAGE), ≥50 Kunitz units/mg protein

CONDITIONS

column

Proteomix SCX-NP1.7, 5 cm x 4.6 mm I.D., 1.7 μm particles (Z777146)

mobile phase

(A) 20 mM sodium phosphate, pH 6.0; (B) A + 1.0 M NaCl

gradient

0 to 75% B in 25 min; 15 min prewash

flow rate

0.75 mL/min

column temp.

ambient

detector

UV, 214 nm

injection

5 μL

sample

1 mg/mL

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