G005889

HPLC Analysis of a Monoclonal Antibody on Zenix^® SEC-150, 30 cm X 4.6 mm I.D., 3 μm particles after Proteolysis with IdeS Protease

application for HPLC

속성

• 기술: HPLC: suitable
• 검사 파라미터: column: Zenix^® SEC-150, 30 cm X 4.6 mm I.D., 3 μm particles (Z777018); mobile phase: 0.15 M potassium phosphate monobasic, pH 7.0 with potassium hydroxide; flow rate: 0.35 mL/min; pressure: 1450 psi (100 bar); column temp.: ambient; detector: UV, 215 nm; injection: 1 μL; sample: digested SO57 mAb, ~0.5 g/L
• 적합성: application for HPLC
• 응용 분야: life science and biopharma

설명

분석 메모

Proteolysis of antibodies can be useful for simplifying further analysis. This application demonstrates separation of the two fragments, F(ab′)2 and scFc, after digestion with IdeS protease

법적 정보

Zenix is a registered trademark of Sepax Technologies