G005683

HPLC Analysis of Caffeine and Taurine on Ascentis^® Express HILIC Column using Evaporative Light Scattering Detection (ELSD, Fast Analysis)

application for HPLC

속성

• 기술: HPLC: suitable
• 검사 파라미터: column: Ascentis Express HILIC, 5 cm x 3.0 mm I.D., 2.7 μm particles (53967-U); mobile phase: [A] 100 mM ammoniumformate, pH 3.0 with formic acid; [B] acetonitrile; (15:85, A:B); flow rate: 0.6 mL/min; column temp.: 25 °C; detector: ELSD, 55 °C, 3.5 bar nitrogen; injection: 2 μL; sample: 1000 μg/mL in 10:90, water: acetonitrile
• 적합성: application for HPLC
• 응용 분야: vitamins, nutraceuticals, and natural products

설명

분석 메모

This application demonstrates the suitability of Ascentis Express HILIC for the efficient separation of caffeine and taurine. Elution is by aqueous normal phase (ANP) as an increase in organic will increase retention.

법적 정보

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany

분석물

Caffeine
Taurine