G005674

HPLC Analysis of Benzodiazepines on Ascentis^® Express Phenyl-Hexyl, Methanol Mobile Phase

application for HPLC

속성

• 기술: HPLC: suitable
• 검사 파라미터: column: Ascentis Express Phenyl-Hexyl, 5 cm x 4.6 mm I.D., 2.7 μm particles (53348-U); mobile phase: [A] water; [B] methanol; (35:65, A:B); flow rate: 1.4 mL/min; pressure: 3600 psi (248 bar); column temp.: 35 °C; detector: UV, 250 nm; injection: 2 μL; sample: 0.1 g/L in 20% methanol
• 적합성: application for HPLC
• 응용 분야: clinical; pharmaceutical (small molecule)

설명

분석 메모

The Phenyl-Hexyl phase of the Ascentis Express product line displays the best selectivity of this sample; selectivity is markedly affected by choice of organic modifier. The application also demonstrates feasibility of fast LC with Ascentis Express by virtue of low backpressure and high efficiency.

법적 정보

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany

분석물

Clonazepam
Oxazepam
Alprazolam
Desmethyldiazepam
Diazepam