821892

ToyoScreen^® Phenyl-600M Process Development Column

phase phenyl, pkg of 6 × 1 mL, 65 μm particle size

• UNSPSC 코드: 41115700

속성

• material: aluminum seal; polypropylene and polyethylene frit
• 제품 라인: ToyoScreen^®
• 분석물 화학적 분류: proteins
• 포장: pkg of 6 × 1 mL
• 제조업체/상표: Tosoh 21892
• 파라미터: ≤50% organic solvent; 0.2-1 mL/min flow rate; 3 bar max. pressure; 4 mL/min max. flow rate
• 기술: HPLC: suitable
• 길이 × I.D.: 3 cm × 6.4 mm
• Matrix: hydroxylated methacrylic polymer
• 기질 활성군: phenyl phase
• 입자 크기: 65 μm
• 작동 pH: 1-13
• 용량: 55 g/L±10 adsorption capacity (lysozyme)
• 분리 기술: hydrophobic interaction (HIC)

설명

일반 설명

Every large scale commercial purification begins at a small scale in a method development lab by evaluating retention and selectivity of the target protein versus impurities, host cell proteins, DNA, etc., on multiple resins, often from different vendors. Depending on the constraints, the process development engineer can either purchase bulk resins to pack several small columns with different resins or take advantage of the speed and convenience of pre-packed columns for resin evaluation. Pre-packed ToyoScreen^® Process Development Columns are available in 1 mL and 5 mL volumes packed with the most popular Toyopearl^® resins. ToyoScreen columns can be used to quickly establish optimum elution conditions. Varying pH, salt type, gradient conditions and flow rate are common experimental parameters explored. Initial results from resin screening and optimization with ToyoScreen columns accurately predict the separation behavior at larger scale.

규격

Clean in place with 0.5 M NaOH or 0.1 M HCl.

추천 제품

Discover LiChropur reagents ideal for HPLC or LC-MS analysis

법적 정보

ToyoScreen is a registered trademark of Tosoh Corporation

Toyopearl is a registered trademark of Tosoh Corporation