추천 제품
product name
apHera™ NH2 HPLC Column, 5 μm particle size, L × I.D. 15 cm × 2 mm
material
stainless steel column
Agency
suitable for USP L82
제품 라인
apHera™
포장
pkg of 1 ea
제조업체/상표
apHera™
파라미터
147 bar pressure (2133 psi)
4-50 °C temperature
기술
HPLC: suitable
LC/MS: suitable
길이 × I.D.
15 cm × 2 mm
기질
polymeric
기질 활성군
amino phase
입자 크기
5 μm
공극 크기
300 Å
작동 pH
1-14
응용 분야
food and beverages
분리 기술
hydrophilic interaction (HILIC)
normal phase
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일반 설명
apHera™ amino columns are based on covalently bonded polyamine specifically optimized for the separation of mono- and oligosaccharides. The elution order mono-, di-, tri-saccharide shows increased eluton volume with increased acetonitrile concentration and complete stability for both acidic and alkaline eluates. The small, robust PVA copolymer bead provides mechanical and chemical strength as well as high column efficiency. Conventional camion columns based on silica do not show long column life, perhaps due to hydrolysis of silica particle by the basic amino group. Since Supelco uses a strong alkaline compatible polymer, these problems are eliminated. Stable retention time and long column life are also characteristic of the column.
Features of Amino apHera™ Columns
Features of Amino apHera™ Columns
- High efficiency for carbohydrate analysis
- Ideal for basic conditions/amphoteric detection
- High selectivity mono- to oligosaccharides
- pH range 2-13
- 300 angstrom pore size
- Stable, predictable retention
- Long column life
법적 정보
apHera is a trademark of Sigma-Aldrich Co. LLC
Choose from one of the most recent versions:
Analytical chemistry, 81(18), 7766-7772 (2009-08-26)
We have developed a practical method for the comprehensive analysis of polar anionic metabolites in biological samples with the use of a nano-LC/MS system. A polyamine-bonded polymer-based apHera NH2 column, which is compatible with ammonium carbonate buffer, effectively retained anionic
Journal of chromatography. A, 1081(2), 174-181 (2005-07-26)
A new procedure based on hydrophilic interaction chromatography coupled to tandem mass spectrometry (ionisation process by pneumatically assisted electrospray in negative ion mode), is developed and validated for the simultaneous determination of underivatised taurine and methionine in beverages rich in
프로토콜
LC/MS Analysis of Glyphosate and Metabolites on apHera™ NH2, 2 mm I.D. Column
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