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53345-U

Supelco

Ascentis® Express 90 Å Phenyl-Hexyl (2.7 μm) HPLC Columns

L × I.D. 10 cm × 3 mm, HPLC Column

동의어(들):

Core-shell (SPP) Fused Core Phenyl HPLC column

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크기 선택

1 EA
₩1,312,598

₩1,312,598

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1 EA
₩1,312,598

About This Item

UNSPSC 코드:
41115700
eCl@ss:
32110501
NACRES:
SB.52

₩1,312,598

구입 가능 여부는 고객센터에 문의하십시오.

제품명

Ascentis® Express Phenyl-Hexyl, 2.7 μm HPLC Column, 2.7 μm particle size, L × I.D. 10 cm × 3 mm

material

stainless steel column

Quality Level

100

Agency

suitable for USP L11

제품 라인

Ascentis®

특징

endcapped

제조업체/상표

Ascentis®

포장

1 ea of

파라미터

60 °C temp. range
600 bar max. pressure (9000 psi)

기술

HPLC: suitable
LC/MS: suitable
UHPLC-MS: suitable
UHPLC: suitable

길이 × I.D.

10 cm × 3 mm

표면적

135 m2/g

불순물

<5 ppm metals

Matrix

Fused-Core particle platform
superficially porous particle

기질 활성군

phenylhexyl phase

입자 크기

2.7 μm

공극 크기

90 Å

pH 범위

2-9

응용 분야

food and beverages

분리 기술

reversed phase

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관련 카테고리

일반 설명

The Phenyl-Hexyl phase has unique selectivity arising from solute interaction with the aromatic ring and its delocalized electrons. It is complementary (orthogonal) to both C18 and RP-Amide phases because of this unique aromaticity. The Phenyl-Hexyl phase also tend to exhibit good shape selectivity, which may originate from solute multipoint interaction with the planar ring system. More retention and selectivity will often be observed for solutes with aromatic electron-withdrawing groups (fluorine, nitro, etc.) or with a delocalized heterocyclic ring system such as the benzodiazepine compounds.

애플리케이션


  • A fully automated and fast method using direct sample injection combined with fused-core column on-line SPE-HPLC for determination of ochratoxin A and citrinin in lager beers.: This study presents a rapid, automated method for the detection of mycotoxins in beer using high-performance liquid chromatography. The use of the Ascentis® Express Phenyl-Hexyl column enables efficient and effective sample processing, demonstrating its applicability in food safety and quality control (Lhotska et al., 2016).

  • Advantages of core-shell particle columns in Sequential Injection Chromatography for determination of phenolic acids.: This paper explores the benefits of using core-shell particle technology, including Ascentis® Express Phenyl-Hexyl columns, in the analysis of phenolic acids. The study highlights the columns′ enhanced performance and efficiency, making them valuable for chemical analysis in environmental and food samples (Chocholous et al., 2013).

법적 정보

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

가장 최신 버전 중 하나를 선택하세요:

시험 성적서(COA)

Lot/Batch Number
USDW002532
USDW002531
USDW002530
USDW002529
USDW002528

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문서 라이브러리 방문

C M Chavez-Eng et al.
Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 1011, 204-214 (2016-01-17)
An ultra-high performance liquid chromatography/tandem mass spectrometry (UPLC-MS/MS) method for the simultaneous determination of (4S,5R)-5-[3,5-bis (trifluoromethyl)phenyl]-3-{[4'-fluoro-5'-isopropyl-2'-methoxy-4-(trifluoromethyl)biphenyl-2-yl] methyl}-4-methyl-1,3-oxazolidin-2-one (anacetrapib, I) and [(13)C5(15)N]-anacetrapib, II in human plasma has been developed to support a clinical study to determine the absolute bioavailability of I.
Petr Chocholouš et al.
Talanta, 103, 221-227 (2012-12-04)
Currently, for Sequential Injection Chromatography (SIC), only reversed phase C18 columns have been used for chromatographic separations. This article presents the first use of three different stationary phases: three core-shell particle-packed reversed phase columns in flow systems. The aim of
Ivona Lhotská et al.
Analytical and bioanalytical chemistry, 408(12), 3319-3329 (2016-03-20)
A new fast and sensitive method based on on-line solid-phase extraction on a fused-core precolumn coupled to liquid chromatography with fluorescence detection has been developed for ochratoxin A (OTA) and citrinin (CIT) determination in lager beer samples. Direct injection of
Alex D Batista et al.
Talanta, 133, 142-149 (2014-12-02)
On-line sample pretreatment (clean-up and analyte preconcentration) is for the first time coupled to sequential injection chromatography. The approach combines anion-exchange solid-phase extraction and the highly effective pentafluorophenylpropyl (F5) fused-core particle column for separation of eight sulfonamide antibiotics with similar
Dalibor Šatínský et al.
Talanta, 142, 124-130 (2015-05-25)
A new on-line SPE-HPLC method using fused-core columns for on-line solid phase extraction and large volume sample injection for increasing the sensitivity of detection was developed for the determination of insecticides fenoxycarb and cis-, trans-permethrin in surface waters. The separation
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문서

Teriparatide Peptide Mapping Analysis by LC-UV-MS

LC-UV-MS workflow details teriparatide peptide mapping, including enzymatic digestion, separation conditions, and QTOF mass spectrometer identification.

Ascentis® Express Phenyl Hexyl Columns for U/HPLC

Improve your reversed phase selectivity for polar aromatics and heterocyclic compounds with the Ascentis® Express Phenyl-Hexyl U/HPLC column.

질문

1–10 / 10 질문  

  1. How can I measure my instrument bandwidth (IBW) and determine what Ascentis® Express HPLC Columns can be used with minimal efficiency loss created by too much internal instrument volume?

    1 답변

    1. The Guide to Dispersion Measurement has simple instructions on how to measure IBW and can be found at sigma-aldrich.com/express.

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  2. Can I use Ascentis Express on any type of HPLC system?

    1 답변

    1. Ascentis Express HPLC columns are capable of use on standard HPLC systems as well as UHPLC systems.  Columns are packed in high pressure hardware capable of withstanding the pressures used in UHPLC systems.

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  3. Can Ascentis® Express HPLC Columns be used for LC-MS?

    1 답변

    1. Express Fused-Core™ particles were designed with LC-MS in mind. Even extremely short column lengths exhibit sufficient plate counts to show high resolving power. The flat van Deemter plots permit resolution to be maintained at very high flow rates to maximize sample throughput. All Ascentis stationary phases have been evaluated for MS compatibility during their development, and the Express phases are no exception. You can expect extremely low column bleed and background while maintaining longest possible column lifetime. A bonus of Ascentis Express columns for high throughput UHPLC and LC-MS is that they are extremely rugged and highly resistant to plugging, a very common failure mode for competitor columns.

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  4. What is the Department of Transportation shipping information for this product?

    1 답변

    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

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  5. What flow rate should I use with Ascentis® Express HPLC Columns?

    1 답변

    1. Based on the minimum in the van Deemter curves, higher flows than 5um particle columns are required in order to maximize Ascentis Express column efficiency. The suggested starting point for flow rate for Ascentis Express columns: 1.6 mL/min for 4.6 mm ID;  0.8 mL/min for 3.0 mm ID; and 0.4mL/min for 2.1 mm ID.

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  6. Can I use Ascentis Express on a UHPLC system?

    1 답변

    1. Yes.  Ascentis Express columns are packed in a way making them suitable for these ultra high pressure instruments.  In fact, Ascentis Express outperforms sub-2 μm micron columns on many applications since Ascentis Express provides the benefits of sub-2 μm particles but at much lower back pressure.  These benefits include the capability of providing fast HPLC and higher resolution chromatography.  The Fused-Core particle consists of a 1.7 μm solid core and a 0.5 μm porous shell.  A major benefit of the Fused-Core particle is the small diffusion path (0.5 μm) compared to conventional fully porous particles. The shorter diffusion path reduces axial dispersion of solutes and minimizes peak broadening.

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  7. Is there anything special I need to do to my HPLC system to use Ascentis Express?

    1 답변

    1. Nothing special is required to use Ascentis Express HPLC columns. To obtain the full benefits of Ascentis Express, one should minimize dispersion or instrument bandwidth in the HPLC system (tubing, detector flow cell) as well as confirm the detector response system is set at a fast level. For more information, request Guidelines for Optimizing Systems for Ascentis Express Columns (T407102)

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  8. What is the pressure rating for Ascentis® Express Phenyl-Hexyl HPLC Columns?

    1 답변

    1. This Ascentis Express column is stable to operating pressures up to 600 bar (9000 psi).

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  9. Do I need special fittings and tubing to connect Ascentis® Express HPLC Columns?

    1 답변

    1. While operating pressures may not exceed the 400 bar (6,000 psi) capability of your traditional instruments, sustained pressures of about 200 bar (3,000 psi) will exceed the recommended pressure for conventional PEEK tubing and fittings at the column inlet. We recommend changing to stainless steel fittings in all high pressure locations and have designed special High Performance HPLC Fittings/Interconnects that will stay tight at pressures of 1,000 bar (15,000 psi) or greater, even when elevated column temperatures are employed.

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  10. Can a 100% aqueous mobile phase be used with the Phenyl-Hexyl HPLC column?

    1 답변

    1. The phenyl-hexyl phase is 100% aqueous compatible.

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