ZRB1308

Anti-Myosin Heavy Chain 7 Antibody, clone 1B19, ZooMAb^® Rabbit Monoclonal

recombinant, expressed in HEK 293 cells

• 동의어(들): MyHC-beta, MyHC-slow, Myosin heavy chain cardiac muscle beta isoform, Myosin heavy chain slow isoform, Myosin-7
• UNSPSC 코드: 12352203
• NACRES: NA.43

속성

• 생물학적 소스: rabbit (recombinant)
• Quality Level: 200
• 재조합: expressed in HEK 293 cells
• 결합: unconjugated
• 항체 형태: purified antibody
• 항체 생산 유형: primary antibodies
• 클론: 1B19, monoclonal; recombinant monoclonal
• 제품 라인: ZooMAb^® learn more
• 양식: lyophilized
• 분자량: calculated mol wt 223.10 kDa
• 종 반응성: rat, mouse, human
• 포장: antibody small pack of 25 μL
• 환경친화적 대안 제품 특성: Waste Prevention; Designing Safer Chemicals; Design for Energy Efficiency; Learn more about the Principles of Green Chemistry.
• 향상된 검증: recombinant expression; Learn more about Antibody Enhanced Validation
• sustainability: Greener Alternative Product
• 기술: ELISA: suitable; affinity binding assay: suitable; immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable; western blot: suitable
• 동형: IgG
• UniProt 수납 번호: P12883
• 환경친화적 대안 카테고리: Aligned,
• 배송 상태: ambient
• 저장 온도: 2-8°C
• 타겟 번역 후 변형: unmodified
• 유전자 정보: human ... MYH7(4625) ; mouse ... Myh7(140781) ; rat ... Myh7(29557)

설명

일반 설명

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies.

Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.

Learn more about ZooMAb here.

특이성

Clone 1B19 is a ZooMAb rabbit recombinant monoclonal antibody that specifically detects Myosin -7. It targets an epitope within 13 amino acids from the N-terminal region.

면역원

KLH-conjugated linear peptide corresponding to 13 amino acids from the N-terminal region of human Myosin heavy chain 7.

애플리케이션

Anti-Myosin Heavy Chain 7, clone 1B19 ZooMAb, Cat. No. ZRB1308, is a recombinant rabbit monoclonal antibody that specifically detects Myosin Heavy Chain 7 and has been tested for use in Affinity Binding Assay ELISA, Immunohistochemistry (Paraffin), and Western Blotting.

ELISA Analysis: 0.5 μg/mL from a representative lot of Anti-Myosin Heavy Chain 7, clone 1B19 ZooMAb Rabbit Monoclonal detected Myosin Heavy Chain 7, but not any other peptide from 6 other proteins.Immunohistochemistry (Paraffin) Analysis: A 1:100-1,000 dilution from a representative lot detected Myosin Heavy Chain 7 in human skeletal muscle and mouse skeletal muscle tissue sections.Affinity Binding Assay: A representative lot of this antibody bound Myosin Heavy Chain 7 with a KD value of 2.51 x 10-7 in an affinity binding assay. Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

표적 설명

Myosin-7 (UniProt: P12883; also known as Myosin heavy chain 7, Myosin heavy chain slow isoform, MyHC-slow, Myosin heavy chain cardiac muscle beta isoform, MyHC-beta) is encoded by the MYH7 (also known as MYHCB) gene (Gene ID: 4625) in human. Myosins are actin-based motor molecules with ATPase activity essential for muscle contraction. Myosins form regular bipolar thick filaments that, together with actin thin filaments, constitute the fundamental contractile unit of skeletal and cardiac muscle. Muscle myosin is a hexameric protein that consists of 2 heavy chain subunits (MHC), 2 alkali light chain subunits (MLC) and 2 regulatory light chain subunits (MLC-2). Fast and slow mammalian muscle myosins differ in their heavy chain sequences. Muscles expressing the two isoforms contract at markedly different velocities. Myosin-7 is a component of thick filaments of the myofibrils. It contains actin- and ATP-binding sites in its conserved catalytic head domain. Mutations in MYH7 gene are known to cause familial hypertrophic cardiomyopathy that is characterized by ventricular hypertrophy leading to dyspnea, syncope, collapse, palpitations, and chest pain. This ZooMAb recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Iorga, B., et al. (2007). J. Biol. Chem. 282(6); 3559-3670).

물리적 형태

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.

재구성

Reconstitute lyophilized antibody pellet with 25μL of ultrapure water or Phosphate Buffered Saline (PBS). Please refer to our reconstitution protocol and the specific application guidance on the suggested starting dilutions and sample type.

저장 및 안정성

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

법적 정보

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

안전성 정보

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 2
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable