Z702722

Nitric Oxide Protocols, 2nd ed.

• ISBN-10: 1-58829237-1
• ISBN-13: 978-1-58829237-7
• UNSPSC 코드: 55101509

속성

• 출판물 정보: A. Hassid, ed., Humana Press, 2004, 260 pp., hard cover

설명

일반 설명

A collection of cutting-edge techniques for measuring nitric oxide and the enzyme that produces it in biological tissues and fluids. These readily reproducible methods can be used to measure novel nitric oxide-related products such as protein nitration and nitrosation, as well as to express nitric oxide synthase in basic research and gene therapy using viral vectors. This book offers a wealth of important practical information that will be of inestimable value both to newcomers and experienced research groups along with being an essential resource for every laboratory involved in NO-related work

목차

1. Measurement of Nitric Oxide by Reconversion of Nitrate/Nitrite to NO
2. High Resolution Capillary Electrophoresis of Nitrite and Nitrate in Biological Samples
3. Nitrite and Nitrate Measurements in Human Urine by Capillary Electrophoresis
4. In Vivo Nitric Oxide Measurements Using a Microcoaxial Electrode
5. Measurement of Exhaled Nitric Oxide
6. Nitric Oxide Imaging in Neurons Using Confocal Microscopy
7. Measurement of Nitric Oxide Synthase Activity In Vivo and in Vitro by Gas Chromatography - Mass Spectrometry
8. A Nonradioactive Assay for Nitric Oxide Synthase Activity in Tissue Extracts
9. Measurement of Nitric Oxide Related Enzymes in the Brain by In Situ Hybridization
10. Real Time Polymerase Chain Reaction to Quantify mRNA for Endothelial Nitric Oxide Synthase
11. Dioxygen Dependent Metabolism of Nitric Oxide
12. Proteomic Method for Identification of Tyrosine Nitrated Proteins
13. Immunohistochemical Detection of S-Nitrosylated Proteins
14. Gene Therapy Techniques for the Delivery of Endothelial Nitric Oxide Synthase to the Corpora Cavernosa for Erectile Dysfunction
15. Adenovirus Mediated Nitric Oxide Synthase Gene Transfer Into the Nucleus Tractus Solitarius in Conscious Rats
16. Gene Therapy in Cancer Via Use of a Retrovector Having a Tumor Specificity and Expressing Inducible Nitric Oxide Synthase
17. Gene Transfer of Nitric Oxide Synthase Via the Use of Adeno-Associated Virus Vectors
18. Adenovirus Mediated Nitric Oxide Synthase Gene Transfer