W0393

Anti-WRN antibody, Mouse monoclonal

clone 195C, purified from hybridoma cell culture

• 동의어(들): Anti-RECQ3, Anti-RECQL2, Anti-RECQL3, Anti-Werner syndrome helicase, Anti-Werner syndrome protein
• MDL number: MFCD02264220
• UNSPSC 코드: 12352203
• NACRES: NA.41

속성

• 생물학적 소스: mouse
• 결합: unconjugated
• 항체 형태: purified immunoglobulin
• 항체 생산 유형: primary antibodies
• 클론: 195C, monoclonal
• 형태: buffered aqueous solution
• 분자량: antigen ~167 kDa
• 종 반응성: human
• 농도: ~1 mg/mL
• 기술: immunohistochemistry: suitable; immunoprecipitation (IP): suitable; indirect ELISA: suitable; western blot: 2-4 μg/mL using nuclear cell extract of HeLa cells
• 동형: IgG1
• UniProt 수납 번호: Q14191
• 배송 상태: dry ice
• 저장 온도: −20°C
• 타겟 번역 후 변형: unmodified
• 유전자 정보: human ... WRN(7486)

설명

일반 설명

Anti-WRN antibody, Mouse monoclonal, (mouse IgG1 isotype) is derived from the hybridoma 195C produced by the fusion of mouse myeloma cells (p3-NS1/Ag4-1) and splenocytes from BALB/c mice immunized with a recombinant fusion protein fragment of human WRN. Werner syndrome ATP-dependent helicase (WRN) is located on chromosome 8 and is 167 kDa. The WRN protein belongs to the RecQ helicase family of DNA helicases.

면역원

recombinant fusion protein fragment of human WRN (amino acids 1074-1432).

애플리케이션

Anti-WRN antibody has been used in

• enzyme linked immunosorbent assay (ELISA)
• immunoblotting
• immunoprecipitation
• immunohistochemistry
• western blotting

생화학적/생리학적 작용

Werner syndrome ATP-dependent helicase (WRN) protein has been demonstrated to possess three known catalytic activities: 3′-5′ helicase, exonuclease and ATPase activities. The C-terminal region of the protein contains a nuclear localization signal (NLS), thus targeting WRN protein to the nucleus, nucleolus and nucleoplasmic foci. The involvement of WRN in multiple DNA metabolic process suggests its function as a tumor suppressor. Mutations in the WRN protein results in Werner syndrome (WS). The epigenetic inactivation of WRN leads to loss of WRN-exonuclease activity, resulting in increased chromosomal instability and hypersensitivity to chemotherapeutic drugs.

물리적 형태

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

안전성 정보

• Storage Class Code: 10 - Combustible liquids
• WGK: nwg
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable
• 개인 보호 장비: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)