U211150

DMT-2′O-Methyl-rU Phosphoramidite

configured for ÄKTA^® and OligoPilot^®

• 동의어(들): 5′-O-[bis(4-methoxyphenyl)phenylmethyl]-2′-O-methyl-uridine, 3′-[2-cyanoethyl N,N-bis(1-methylethyl)phosphoramidite], DMT-2′O-Methyl-rU amidite
• 실험식(Hill 표기법): C₄₀H₄₉N₄O₉P
• CAS Number: 110764-79-9
• Molecular Weight: 760.81
• MDL number: MFCD00792626
• UNSPSC 코드: 41116105
• PubChem Substance ID: 329827609
• NACRES: NA.51

속성

• 생물학적 소스: non-animal source (no BSE/TSE risk)
• Quality Level: 300
• 제품 라인: Proligo Reagents
• 분석: ≥99% (³¹P-NMR); ≥99.0% (reversed phase HPLC)
• 형태: powder
• 기술: oligo synthesis: suitable
• 불순물: ≤0.1% single unspecified Impurity (reversed phase HPLC); ≤0.3% mU2 (reversed phase HPLC, Hydrolysate); ≤0.3% mU3 (reversed phase HPLC, DMT-rme); ≤0.3% water content (Karl Fischer); ≤0.5% P(III) Impurities 100-169ppm (³¹P-NMR); ≤1.0% mU1 (reversed phase HPLC, DMT-rUme-DMT); ≤3% residual Solvent content
• 색상: white to off-white
• λ: conforms (UV/VIS Identity)
• 적합성: conforms to structure for H-NMR; conforms to structure for LC-MS
• 호환성: configured for ÄKTA^® and OligoPilot^®
• 뉴클레오시드 프로파일: base: uridine; base protecting group: none; 2' protecting group: methyl; 5' protecting group: DMT; deprotection: fast/standard
• 저장 온도: 2-8°C
• SMILES string: CO[C@@H]1[C@H](OP(OCCC#N)N(C(C)C)C(C)C)[C@@H](COC(c2ccccc2)(c3ccc(OC)cc3)c4ccc(OC)cc4)O[C@H]1N5C=CC(=O)NC5=O
• InChI: 1S/C40H49N4O9P/c1-27(2)44(28(3)4)54(51-25-11-23-41)53-36-34(52-38(37(36)49-7)43-24-22-35(45)42-39(43)46)26-50-40(29-12-9-8-10-13-29,30-14-18-32(47-5)19-15-30)31-16-20-33(48-6)21-17-31/h8-10,12-22,24,27-28,34,36-38H,11,25-26H2,1-7H3,(H,42,45,46)/t34-,36-,37-,38-,54?/m1/s1
• InChI key: UVUOJOLPNDCIHL-XKZJCBTISA-N

설명

일반 설명

DMT-2′O-Methyl-rU Phosphoramidite belongs to the class of 2′O-Methyl RNA Phosphoramidites.

애플리케이션

2′O-Methyl RNA nucleoside including DMT-2′O-Methyl-rU Phosphoramidite can be advantageously incorporated in nucleic acid probes with RNA or DNA for in-vivo or in-vitro applications to convey nuclease resistance.

특징 및 장점

Its key features include:

• High yield of crude oligonucleotides
• Compatible with DNA synthesis
• Can be employed together with DNA or RNA phosphoramidites in the same synthesis to produce mixmer oligonucleotides
• Recommended deprotection conditions are 8 hours at 55 °C using concentrated ammonia solution, or with AMA (concentrated ammonia/ 40% aqueous methylamine I/I, v/v) for 10 minutes at 65 °C
• Purification and other downstream processing of fully modified 2′OMethyl RNA oligonucleotides are simpler than in the case of RNA, as no special precautions are required to provide protection against nucleolytic degradation
• Synthesis of 2′O-Methyl RNA oligonucleotides is similar to standard DNA synthesis but requires an elongated coupling time (recommended is 6 minutes compared to 90 seconds for DNA monomers)
• 2′O-Methyl RNA phosphoramidites are also available with fast deprotection chemistry

기타 정보

The unique combination of properties of 2′O-Methyl RNA had found widespread use in the fields of:

• Diagnostic probes
• Aptamer and ribozyme development
• Mixed 2′O-Methyl-RNA/DNA antisense molecules

법적 정보

OligoPilot is a registered trademark of Cytiva

ÄKTA is a registered trademark of Cytiva

안전성 정보

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable