추천 제품
설명
wavelength 500-750nm
사용
sufficient for ~50 manual assays
단백질 검출 수준 측정 범위
0-1 mg/mL
파라미터
18-26 °C temperature
200 μL sample volume
저장 온도
2-8°C
일반 설명
The biuret and Lowry procedures are used for protein determination. The former is widely used for clinical assays. The latter, though more sensitive, is used for investigative work and is limited by poor stability of combined reagents, non-reproducibility of color, especially at low protein concentration, and nonlinear chromogenic response with protein concentration. Ohnishi and Barr modified the biuret reagent for the Lowry procedure, thereby simplifying it while improving the stability of the combined reagent.
애플리케이션
Total Protein Kit, Micro-Lowry, Onishi & Barr Modification has been used to determine total soluble protein concentrations:
- in the dialyzates/extracts of gliadins
- in the extracts of flour (Avena sativa and Triticum durum) and seeds (Chenopodium quinoa, Salvia hispanica L.)
- in the stored samples of rat liver microsomal fractions
원리
Colorimetric, Endpoint.assay. Ohnishi and Barr′s modification of micro Lowry method. According to procedure, dilute biuret reagent reacts with peptide bonds to yield a purple-blue complex, the color of which is intensified by the addition of phenol reagent. Absorbance, read at 550-750 nm, is used to determine results from a standard curve.
키트 구성품 역시 별도로 이용 가능함
제품 번호
설명
SDS
- B3934Biuret reagent, protein detection level 150-1,000 μg/mL 110 mLSDS
- Folin & Ciocalteu’s phenol reagent 5 mL
필요하나 제공되지 않음
제품 번호
설명
가격
신호어
Danger
유해 및 위험 성명서
Hazard Classifications
Eye Dam. 1 - Met. Corr. 1 - Skin Corr. 1
Storage Class Code
8A - Combustible corrosive hazardous materials
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
시험 성적서(COA)
제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.
Inge Everaert et al.
American journal of physiology. Renal physiology, 318(4), F1030-F1040 (2020-03-10)
Manipulation of circulating histidine-containing dipeptides (HCD) has been shown to affect the development of diabetes and early-stage diabetic nephropathy (DN). The aim of the present study was to investigate whether such interventions, which potentially alter levels of circulating HCD, also
Fei-Fan Cai et al.
Journal of biomedical materials research. Part B, Applied biomaterials, 109(8), 1090-1104 (2020-12-06)
The concept of adding inorganic fillers into hydrogels to form hydrogel nanocomposites often provides advantageous properties which can be exploited for successful 3D biofabrication. In this study, a new composite hydrogel combining oxidized alginate-gelatin (ADA-GEL) hydrogel and Laponite® nanoclay as
A Kaliszewska et al.
Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association, 95, 89-95 (2016-07-06)
Cereal proteins are of clinical interest because of their cytotoxic and immunogenic features being associated to allergic processes and intestinal disorders. In addition to gliadins, there has been suggested an important role for non-gluten modulating factors (nGMF) on the onset
Method for Detecting NADPH-Cytochrome P450 Reductase in Liver Microsomal Fractions by Using Native Polyacrylamide Gel Electrophoresis and NADPH-Diaphorase Staining
Yokoyama H, et al.
American Journal of Analytical Chemistry (2013)
David Kessel
Photochemistry and photobiology, 90(5), 1211-1213 (2014-04-26)
When the initial effect of photodynamic therapy (PDT) involves mitochondrial photodamage, an early effect is loss of the mitochondrial membrane potential (ΔΨm ). Using murine hepatoma 1c1c7 cells and a photosensitizing agent known to target mitochondria, we examined loss of
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