T5944

Tumor Necrosis Factor-α from rat

TNF-α, recombinant, expressed in E. coli, powder, suitable for cell culture

• 동의어(들): Cachectin, TNF-α
• MDL number: MFCD00282383
• UNSPSC 코드: 12352202
• NACRES: NA.77

속성

• 생물학적 소스: rat
• Quality Level: 200
• 재조합: expressed in E. coli
• 분석: ≥98% (SDS-PAGE and HPLC)
• 형태: powder
• 품질: endotoxin tested
• 분자량: predicted mol wt ~17 kDa
• 포장: pkg of 10 μg; pkg of 50 μg
• 저장 조건: avoid repeated freeze/thaw cycles
• 기술: cell culture | mammalian: suitable
• 불순물: <1 EU/μgtested (LAL test)
• 색상: white
• solubility: water: soluble
• UniProt 수납 번호: P16599
• 저장 온도: −20°C
• 유전자 정보: rat ... Tnf(24835)

설명

일반 설명

The Tnf (tumor necrosis factor) gene is mapped to rat chromosome 20p12.

애플리케이션

TNF-α influences the growth and function of both normal and neoplastic cells. TNF-α causes cytolysis or cytostasis of certain transformed cells being synergistic with γ-interferon in its cytotoxicity.

Tumor Necrosis Factor-α from rat has been used:

• To stimulate inducible nitric oxide synthase (iNOS) expression in the macrophages as an indication of M1 macrophage activation
• To study its effect on pancreatic β cell apoptosis
• To evaluate the effect of progesterone on the expression of tumor necrosis factor (TNF)-α in synovial membrane
• As a blocking antigen in the control, for immunohistochemical analysis

생화학적/생리학적 작용

Tumor necrosis factor-α (TNF-α), also known as cachectin, plays roles in antitumor activity, immune modulation, inflammation, anorexia, cachexia, septic shock, viral replication, and hematopoiesis. TNF-α is expressed by a variety of cells, with numerous inductive and suppressive agents. It is primarily produced by macrophages in response to immunological challenges such as bacteria (lipopolysaccharides), viruses, parasites, mitogens, and other cytokines. TNF-α is cytotoxic for many transformed cells (its namesake activity) but in normal diploid cells, it can stimulate proliferation (fibroblasts), differentiation (myeloid cells) or activation (neutrophils). TNF-α also shows antiviral effects against both DNA and RNA viruses and it induces production of several other cytokines. TNF-α and the related molecule TNF-β (LT-α) share close structural homology with 28% amino acid sequence identity and both activate the same TNF receptors, TNFR1 and TNFR2.

물리적 형태

Lyophilized from an 0.2 μm-filtered buffered solution with bovine serum albumin as a carrier.

분석 메모

The biological activity is measured in a cytotoxic assay using a TNF-susceptible mouse L929 cell line in the presence of actinomycin D.

안전성 정보

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable