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Merck
모든 사진(3)

문서

T1325

Sigma-Aldrich

Anti-Phosphotyrosine antibody produced in rabbit

0.5 mg/mL, affinity isolated antibody, buffered aqueous solution

동의어(들):

Anti-p-Tyr

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About This Item

MDL number:
UNSPSC 코드:
12352203
NACRES:
NA.44

생물학적 소스

rabbit

결합

unconjugated

항체 형태

affinity isolated antibody

항체 생산 유형

primary antibodies

클론

polyclonal

형태

buffered aqueous solution

농도

0.5 mg/mL

기술

indirect immunofluorescence: 3-6 μg/mL using A431 stimulated by human EGF.
western blot: 0.3-0.6 μg/mL using total cell extract of A431 stimulated by human EGF

배송 상태

dry ice

저장 온도

−20°C

타겟 번역 후 변형

phosphorylation (pTyr)

특이성

Anti-Phosphotyrosine recognizes tyrosine phosphorylated proteins.

면역원

phospho-L-tyrosine conjugated to KLH

애플리케이션

Anti-Phosphotyrosine antibody is suitable for use in indirect immunofluorescence (3-6 μg/mL using A431 stiμLated by human EGF) and immunoblot (using U2OS Dr1 cells ). The antibody may also be used for immunoprecipitation (1:40 using rat lumbar and thoracic lysates) and immunocytochemistry.

생화학적/생리학적 작용

Protein phosphorylation is the most abundant among post-translational modifications of cellular proteins, regulating intracellular signal transduction pathways in every living cell. Tyrosine, serine and threonine are the major amino acids that are phosphorylated in proteins. Antibodies specific for phosphotyrosine are essential tools for the characterization of tyrosine phosphorylation in many signal transduction pathways.

물리적 형태

Solution in 0.01 M phosphate buffered saline, pH 7.4, and 1% BSA, containing 15 mM sodium azide.

저장 및 안정성

For extended storage, freeze in working aliquots. Repeated freezing and thawing is not recommended. Storage in "frost-free" freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

12 - Non Combustible Liquids

WGK

nwg

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable


시험 성적서(COA)

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문서 라이브러리 방문

A Wiedłocha et al.
Molecular and cellular biology, 16(1), 270-280 (1996-01-01)
U2OS Dr1 cells, originating from a human osteosarcoma, are resistant to the intracellular action of diphtheria toxin but contain toxin receptors on their surfaces. These cells do not have detectable amounts of fibroblast growth factor receptors. When these cells were
Ying Xu et al.
Carcinogenesis, 37(2), 215-222 (2016-01-01)
Dysregulated expression of epidermal growth factor receptor (EGFR) has been implicated in many cancer events, while peroxisome proliferator-activated receptor γ (PPARγ) negatively regulates cancer progression. The molecular mechanism of EGFR interaction with PPARγ is still unclear. Here, we found that
Shuangsong Hong et al.
The Journal of biological chemistry, 279(28), 29341-29350 (2004-05-05)
Diabetic neuropathy is a common form of peripheral neuropathy, yet the mechanisms responsible for pain in this disease are poorly understood. Alterations in the expression and function of voltage-gated tetrodotoxin-resistant (TTX-R) sodium channels have been implicated in animal models of
Rafael S Demarco et al.
Methods (San Diego, Calif.), 68(1), 218-227 (2014-05-07)
The process of spermatogenesis in Drosophila melanogaster provides a powerful model system to probe a variety of developmental and cell biological questions, such as the characterization of mechanisms that regulate stem cell behavior, cytokinesis, meiosis, and mitochondrial dynamics. Classical genetic
Michael Bachmann et al.
Journal of cell science, 135(7) (2022-03-29)
Integrin-mediated adhesions are convergence points for multiple signaling pathways. Their inner structure and diverse functions can be studied with super-resolution microscopy. Here, we examined the spatial organization within focal adhesions by analyzing several adhesion proteins with structured illumination microscopy (SIM).

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