T1200

Anti-Tal (FQ-17) antibody produced in rabbit

affinity isolated antibody, buffered aqueous solution

• 동의어(들): Anti-Tsg101-associated ligase
• MDL number: MFCD06798551
• UNSPSC 코드: 12352203
• NACRES: NA.41

속성

• 생물학적 소스: rabbit
• 결합: unconjugated
• 항체 형태: affinity isolated antibody
• 항체 생산 유형: primary antibodies
• 클론: polyclonal
• 양식: buffered aqueous solution
• 분자량: antigen 84 kDa
• 종 반응성: human, mouse, rat
• 기술: immunoprecipitation (IP): 20-40 μg using mouse brain S1 cytosolic fraction; indirect immunofluorescence: 10-20 μg/mL using mouse fibroblast NIH3T3 cell line; microarray: suitable; western blot: 2-4 μg/mL using whole extract of human kidney 293 cells expressing human Tal
• UniProt 수납 번호: Q6UWE0
• 배송 상태: dry ice
• 저장 온도: −20°C
• 타겟 번역 후 변형: unmodified
• 유전자 정보: human ... LRSAM1(90678) ; mouse ... Lrsam1(227738)

설명

일반 설명

The gene LRSAM1 (leucine-rich repeat and sterile a motif-containing protein 1) is mapped to human chromosome 9q33.3. The protein is predicted to contain a leucine-rich repeat (LRR), an ezrin-radixin-moezin (ERM) domain, a coiled-coil (CC) region, a SAM (sterile α motif) domain and a carboxyl-terminal C3HC4-type RING (really interesting new gene) finger domain.

면역원

synthetic peptide encoding amino acids 5-21 located near the N-terminus of human Tal, conjugated to KLH. This sequence is identical in mouse and rat Tal.

생화학적/생리학적 작용

LRSAM1 (leucine-rich repeat and sterile a motif-containing protein 1) is an E3 ligase. It binds and ubiquitinates Tsg101 (tumor susceptibility gene 101), inactivating Tsg101-mediated sorting of epidermal growth factor receptors and viral proteins. It also protects cytoplasm from invasive pathogens by participating in ubiquitination associated with intracellular bacteria. Mutations in LRSAM1 are linked with Charcot-Marie-Tooth disease.

물리적 형태

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.