추천 제품
생물학적 소스
human
재조합
expressed in CHO cells
분석
≥95% (HPLC)
≥95% (SDS-PAGE)
형태
lyophilized
분자량
24.4 kDa
포장
pkg of 20 μg
불순물
endotoxin, tested
UniProt 수납 번호
배송 상태
wet ice
저장 온도
−20°C
유전자 정보
human ... RSPO2(340419)
관련 카테고리
일반 설명
Recombinant human R-Spondin-2 (R-Spo2) is a 24.4kDa protein consisting of 212 amino acid residues. R-Spo2 is encoded by the gene mapped to human chromosome 8q23.1. The encoded protein belongs to the R-spondin family of secreted proteins. Members of RSpo family contain two furin-like cysteine-rich domains at N- terminal end and thrombospondin structural domain and a basic charged C- terminal domain.
생화학적/생리학적 작용
R-Spondin 2 (R-Spo2) plays a vital role in the activation of canonical Wnt signaling pathway, which is essential for normal morphogenesis of the respiratory tract and limbs. R-Spo2 positively regulates Wnt-dependent mineralization of osteoblasts, myogenic differentiation and hypertrophic myotube formation. R-Spo2 is also involved in skeletal development. Aberration in the expression of RSPO2 causes ossification of the posterior longitudinal ligament of the spine (OPLL), resulted by the altered differentiation of spinal ligament MSCs (mesenchymal stem cells) into chondrocytes. R-Spo2 is associated with various developmental processes, such as myogenesis, craniofacial morphogenesis, keratinocyte proliferation, osteoblast maturation, lung and limb development. R-Spo2 interacts with leucine-rich-repeat-containing G-protein-coupled receptor 5 (Lgr5) and stimulates acetylcholine receptor (AChR) clustering at the neuromuscular junction (NMJ).
물리적 형태
Lyophilized from 10 mM Sodium Phosphate, pH 7.5 + 150 mM NaCl.
재구성
Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-0.5 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
시험 성적서(COA)
제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.
R-spondin 2 is required for normal laryngeal-tracheal, lung and limb morphogenesis.
Bell SM
Development, 135(6), 1049-1058 (2008)
R-Spondin family members regulate the Wnt pathway by a common mechanism.
Kim KA
Molecular Biology of the Cell, 19(6), 2588-2596 (2008)
Secreted factor R-Spondin 2 is involved in refinement of patterning of the mammalian cochlea.
Mulvaney JF
Developmental Dynamics, 242(2), 179-188 (2013)
R-spondin 2 promotes acetylcholine receptor clustering at the neuromuscular junction via Lgr5.
Nakashima H
Scientific Reports, 6:28512 (2016)
Minke E Binnerts et al.
Proceedings of the National Academy of Sciences of the United States of America, 104(37), 14700-14705 (2007-09-07)
The R-Spondin (RSpo) family of secreted proteins act as potent activators of the Wnt/beta-catenin signaling pathway. We have previously shown that RSpo proteins can induce proliferative effects on the gastrointestinal epithelium in mice. Here we provide a mechanism whereby RSpo1
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