SRP0733

PCTK2 (CDK17)/CYCLINY, Active

PRECISIO^® Kinase, recombinant

• UNSPSC 코드: 12352202
• NACRES: NA.77

속성

• 재조합: expressed in baculovirus infected Sf9 cells
• 분석: 70-95% (SDS-PAGE)
• 양식: frozen liquid
• 특이 활성도: 15-25 nmol/min·mg (radiometric)
• 분자량: ~64 kDa (Cyclin Y); ~88 kDa (PCTK2)
• 농도: 0.1 μg/μL
• 수납 번호(accession number): NM_002595; NM_145012
• 배송 상태: dry ice
• 저장 온도: −70°C
• 유전자 정보: human ... CCNY(219771) , CDK17(5128)

설명

애플리케이션

Useful for kinase assay and Western blot.

생화학적/생리학적 작용

Recombinant full-length human PCTK2 (CDK17) and Cyclin Y (2-end) were co-expressed by Baculovirus in Sf9 insect cells using N-terminal GST tags. PCTK2 (CDK17) or cyclin-dependent kinase 17 belongs to the cdc2/cdkx subfamily of the ser/thr family of protein kinases. PCTK2 has similarity to a rat protein that is thought to play a role in terminally differentiated neurons ⁽¹⁾. The identification of a large family of cdc2-related kinases opens the possibility of combinatorial regulation of the cell cycle together with the emerging large family of cyclins ⁽²⁾.

재구성

Formulated in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, with 25% glycerol.

저장 및 안정성

Store in aliquots at -70°C. Avoid freeze/thaw cycles.

법적 정보

PRECISIO is a registered trademark of Merck KGaA, Darmstadt, Germany

안전성 정보

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 1
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable