SRP0415

PKM1 active human

recombinant, expressed in E. coli, ≥80% (SDS-PAGE)

• 동의어(들): OIP3, PK3, PKM2, TCB, pyruvate kinase, muscle
• CAS Number: 1644286-11-2
• UNSPSC 코드: 12352200
• NACRES: NA.32

속성

• 생물학적 소스: human
• 재조합: expressed in E. coli
• 분석: ≥80% (SDS-PAGE)
• 양식: aqueous solution
• 분자량: 59 kDa
• 포장: pkg of 20 μg
• 제조업체/상표: Sigma-Aldrich
• 저장 조건: avoid repeated freeze/thaw cycles
• 농도: 1.8 mg/mL
• 기술: cell based assay: suitable
• solubility: water: soluble
• NCBI 수납 번호: NM_182470
• UniProt 수납 번호: P14618
• 응용 분야: life science and biopharma
• 배송 상태: dry ice
• 저장 온도: −70°C
• 유전자 정보: human ... PKM(5315)

설명

일반 설명

Research area: Cell Signaling

Pyruvate kinase (PK) has four isozymes such as PKL, PKR, PKM1, and PKM2.[1][2] PKM1/2 is located in the distal convoluted tubule of the kidney, embryo, lungs, proliferative tissues,[2] retina, adipose tissues, and islets.[1] The PKM1 gene is located on the human chromosome at 15q23.[3]

애플리케이션

PKM1active human has been used in:

• cell-based kinase assay to study the ability of a compound to inhibit the kinase activity of PKM1.[4]
• oxidative stress experiments on PKM2.[5]
• Co-immunoprecipitation (Co-IP) to determine pyruvate kinase M2 (PKM2) as a novel direct binding partner of phosphoserine aminotransferase 1 (PSAT1).[6]

생화학적/생리학적 작용

Pyruvate kinase (PK) catalyzes the dephosphorylation of phosphoenolpyruvate(PEP) into pyruvate. It is also involved in glycolysis by releasing ATP.Allosteric effects and intracellular signal transduction regulate the enzymaticactivity of PKM1. PKM1 is involved in several biological processes such asmetabolic reprogramming, and innate and acquired immune response. Itparticipates in protein interactions, protein synthesis, glycolysis, andintranuclear signal transduction.[1]

안전성 정보

• 픽토그램: GHS08,GHS07
• 신호어: Danger
• 유해 및 위험 성명서: H315,H319,H360D
• 예방조치 성명서: P202 - P264 - P280 - P302 + P352 - P305 + P351 + P338 - P308 + P313
• Hazard Classifications: Eye Irrit. 2 - Repr. 1B - Skin Irrit. 2
• Storage Class Code: 6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
• WGK: WGK 1
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable