SRP0290

Cathepsin F Active human

recombinant, expressed in FreeStyle^™ 293-F cells, ≥90% (SDS-PAGE)

• 동의어(들): CATSF, CLN13
• 효소 위원회 번호: 3.4.22.41 (BRENDA, IUBMB)
• UNSPSC 코드: 12352204
• NACRES: NA.54

속성

• 재조합: expressed in FreeStyle^™ 293-F cells
• 분석: ≥90% (SDS-PAGE)
• 형태: aqueous solution
• 특이 활성도: ≥18 pmol/min-μg
• 분자량: 53 kDa
• 기술: activity assay: suitable
• solubility: soluble; water: soluble
• NCBI 수납 번호: NM_003793
• 배송 상태: dry ice
• 저장 온도: −70°C
• 유전자 정보: human ... CTSF(8722)

설명

일반 설명

Research area: CELL SIGNALING

Human cathepsin F (GenBank Accession No. NM_003793), CD33 signal peptide(amino acids 1-16) + cathepsin S (amino acids 20-end), with C-terminal HIS tag, MW = 53 kDa (more than 58 kDa after post-modification), expressed in FreeStyle 293-F cells.

애플리케이션

Active human cathepsin F is useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. Active human cathepsin F has been used in a study to assess its implications for the development of novel immunomodulators. Active human cathepsin F has also been used in a study to investigate microarray expression analysis and identification of serum biomarkers for Niemann-Pick disease.

생화학적/생리학적 작용

Cathepsin F (CtsF) are endopeptidases that play a role in lysosomal protein degradation. Beyond its involvement in protein recycling, CtsF has also been associated with immune responses mediated by Toll-like receptors (TLRs) and antigen processing. Additionally, mutation in the cathepsin F gene is related to the development of familial papillary thyroid cancer , type B Kufs disease, defective lysosomal degradation and abnormal autophagy, and Alzheimer′s disease.

단위 정의

One unit is defined as the amount of enzyme that will cleave 1 pmol of substrate per min at 37°C

물리적 형태

Formulated in 45 mM sodium acetate, 135 mM NaCl and 10% glycerol.

제조 메모

Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.

법적 정보

FreeStyle is a trademark of Invitrogen Corp.

안전성 정보

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable