UbcH13 (GenBank Accession No. NM_003348), amino acids 2-end, with N-terminal His-tag, MW = 17.6 kDa, expressed in an E. coli cell expression system.
애플리케이션
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
물리적 형태
Formulated in 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 50% glycerol and 3 mM DTT.
제조 메모
Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.