SRP0158

Histone H3 (2-58) human

recombinant, expressed in E. coli, ≥70% (SDS-PAGE)

• 동의어(들): HIST1H3E, Histone H3.1
• UNSPSC 코드: 12352200
• NACRES: NA.32

속성

• 생물학적 소스: human
• 재조합: expressed in E. coli
• 분석: ≥70% (SDS-PAGE)
• 양식: aqueous solution
• 분자량: 32 kDa
• 포장: pkg of 500 μg
• 저장 조건: avoid repeated freeze/thaw cycles
• 농도: >0.02 mg/mL
• NCBI 수납 번호: NM_003532
• UniProt 수납 번호: P68431
• 배송 상태: dry ice
• 저장 온도: −70°C
• 유전자 정보: human ... HIST1H3E(8353)

설명

일반 설명

The mammalian chromatin is present in nucleosomes, made of histone octamers (H2A,H2B,H3,H4)₂. Mammals contain three main classes of histone H3 variants: the replicative histones (H3.1 and H3.2), the replacement histone (H3.3) and the centromeric histone (Cenp-A).[1][2][3]
Human Histone 3 (GenBank Accession No. NM_003532), (amino acid 2-58) with N-terminal GST tag, MW = 32kDa, expressed in an Escherichia coli expression system.

애플리케이션

Suitable substrate for histone methyltransferases

생화학적/생리학적 작용

Histone proteins are basic building blocks of chromatin.[4] H3.1 (also referred to as HIST1H3E) is mainly expressed in the S phase and is termed as replication-dependent histone.[5] Selective methylation of H3.1 controls replication in heterochromatin area.[6]

물리적 형태

Formulated in 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 20% glycerol and 3 mM DTT.

제조 메모

Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.