SRP0135

G9a human

recombinant, expressed in E. coli, ≥70% (SDS-PAGE)

• 동의어(들): EHMT2, Euchromatic histone-lysine N-methyltransferase 2, HLA-B-associated transcript 8, KMT1C, Lysine N-methyltransferase 1C
• UNSPSC 코드: 12352200
• NACRES: NA.32

속성

• 생물학적 소스: human
• 재조합: expressed in E. coli
• 분석: ≥70% (SDS-PAGE)
• 양식: aqueous solution
• 분자량: 74.6 kDa
• 포장: pkg of 50 μg
• 농도: >0.02 mg/mL
• NCBI 수납 번호: NM_006709
• UniProt 수납 번호: Q96KQ7
• 배송 상태: dry ice
• 저장 온도: −70°C
• 유전자 정보: human ... EHMT2(10919)

설명

일반 설명

Human G9a (euchromatic histone-lysine N-methyltransferase 2) with N-terminal GST tag, MW = 74.6 kDa, expressed in an E. coli expression system. G9a is a member of SET (Su(var)3-9, enhancer-of-zeste and trithorax) domain containing, Su(var)3-9 family of proteins. This domain is evolutionary conserved.[1]

애플리케이션

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

생화학적/생리학적 작용

The encoded protein G9a (euchromatic histone-lysine N-methyltransferase 2) is a methyltransferase that methylates lysine residues of histone H3.[2] Overexpression of G9a is observed in cancer. It is known to participate in cancer cell migration, invasion and metastasis.[3] G9a is an epigenetic regulator and can inhibit the action of tumor suppressor genes, thereby, contributes to carcinogenesis. Thus, G9a is considered to be an oncogenic and metastatic factor.[4] It also affects DNA damage repair mechanism, which might make tumor cells resistant to radiation and chemotherapy.[5] G9a serves as a target for cancer therapy including breast cancer.[4]

단위 정의

One unit is defined as the amount of enzyme required to methylate 1 pmol of substrate/min at 37°C.

물리적 형태

Formulated in 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 30% glycerol and 3 mM DTT.

제조 메모

Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.

안전성 정보

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 1
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable