SRP0103

HDAC2 FLAG tag human

recombinant, expressed in baculovirus infected insect cells, ≥50% (SDS-PAGE)

• 동의어(들): HD2, Histone deacetylase 2, Transcriptional regulator homolog RPD3, YAF1, YY1-associated factor 1
• UNSPSC 코드: 12352200
• NACRES: NA.77

속성

• 생물학적 소스: human
• 재조합: expressed in baculovirus infected insect cells
• 분석: ≥50% (SDS-PAGE)
• 형태: aqueous solution
• 분자량: 60 kDa
• 포장: pkg of 50 μg
• 저장 조건: avoid repeated freeze/thaw cycles
• 농도: >0.02 mg/mL
• NCBI 수납 번호: Q92769
• UniProt 수납 번호: Q92769
• 배송 상태: dry ice
• 저장 온도: −70°C
• 유전자 정보: human ... HDAC2(3066)

설명

일반 설명

Human HDAC2, GenBank Accession No. Q92769, full length with C-terminal DDDDK tag (FLAG), MW = 60 kDa, expressed in baculovirus expression system.
The HDAC2 (histone deacetylase 2) gene is mapped to human chromosome 6q21 and is ubiquitously expressed.

애플리케이션

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

생화학적/생리학적 작용

Histone deacetylase 2 (HDAC2) is known to regulate DNA damage signalling, maintain genomic stability and prevents tumor formation and progression. Hdac2 does not possess a DNA binding domain, it forms multi-component repressor complexes for its interaction with transcription factors. Hdac2 also mediates cell proliferation, differentiation and apoptosis. HDAC2 controls response to chronic inflammation in COPD (chronic obstructive pulmonary disease). It activates Wnt signaling pathway via β-catenin T-cell transcription factor. Deletion of HDAC2 along with HDAC1 leads to anemia and thrombocytopenia.

단위 정의

One unit is defined as the amount of enzyme required to deacetylate 1 pmol of substrate/min at 37°C.

물리적 형태

Formulated in 25 mM Tris-HCl, pH 8.0, 138 mM NaCl, 0.05% Tween-20 and 10% glycerol.

제조 메모

Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.