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SHM01

Sigma-Aldrich

MISSION® ExpressMag® Super Magnetic Kit

Increases transduction efficiency

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About This Item

UNSPSC 코드:
41106609

제품 라인

MISSION®

유통기한

≥2 yr at ~4 °C

저장 온도

2-8°C

일반 설명

To view protocols from Sigma collaborators for ExpressMag system visit the link below.
www.sigmaaldrich.com/mission-protocols

애플리케이션

MISSION® ExpressMag® Super Magnetic Kit contains all of the components needed to start enhancing your lentiviral transductions. The kit contains a 100 μL vial of ExpressMag™ Beads, enough for 63 wells of a 96 well plate. These beads bind the lentiviral particles and enable the ExpressMag™ magnetic plate to pull the virus down to the surface of cells thereby significantly increasing transduction efficiency. This product can be used in conjunction with standard sized well plates, T-25 through T-75 tissue culture flasks, and up to 5 cm dishes. The kit has been documented to enhance MISSION® lentiviral transduction of suspension 293T as well as allowing the transduction of primary human cells such as keratinocytes at MOI as low as 1. See the technical bulletin for other cell lines transduced with the ExpressMag™ system.

주의사항

Do not autoclave! Clean surface of magnet with 70% isopropyl alcohol.
Magnets should be kept away from magnetic storage devices, such as hard disks, ID cards, and credit cards, and from electronic devices and ferromagnetic materials. Persons with cardiac pacemakers should not work with these magnets.

법적 정보

ExpressMag is a registered trademark of Sigma-Aldrich Co. LLC
MISSION is a registered trademark of Merck KGaA, Darmstadt, Germany

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제품 번호
설명
SDS
  • SHM03MISSION® ExpressMag® Beads, Increases transduction efficiencySDS
  • SHM04MISSION® ExpressMag® Super Magnetic Plate, Increases transduction efficiencySDS

Storage Class Code

10 - Combustible liquids

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

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시험 성적서(COA)

Lot/Batch Number

It looks like we've run into a problem, but you can still download Certificates of Analysis from our 문서 section.

도움이 필요하시면 연락하세요. 고객 지원 부서

이 제품을 이미 가지고 계십니까?

문서 라이브러리에서 최근에 구매한 제품에 대한 문서를 찾아보세요.

문서 라이브러리 방문

문서

Safe Lentivirus Handling

Lentiviral vector systems prioritize safety features, with design precautions preventing replication. Good handling practices are essential for use.

Successful Transduction Using Lentivirus

Get tips for handling lentiviruses, optimizing experiment setup, titering lentivirus particles, and selecting helpful products for transduction.

프로토콜

Protocol for Transducing Mouse Embryonic Fibroblasts (MEF) using MISSION® ExpressMag™ Super Magnetic Kit®

This detailed procedure allows you to transduce Mouse Embryonic Fibroblasts (MEF) using MISSION ExpressMag Super Magnetic Kit.

CRISPR Lentiviral Screening: Antibiotic Selection

Lentivirus versions of genome modification technologies support successful CRISPR, RNAi, and ORF experiments.

FACS Titration of Lentivirus Protocol

FACS sorts cells based on light scattering and fluorescence for objective cell analysis.

질문

1–6 / 6 질문  

  1. What is the typical fold increase in transduction efficiency when using ExpressMag beads?

    1 답변

    1. The transduction efficiency increase observed when using the ExpressMag products will depend on the cell line. It is reasonable to expect a roughly 10-fold or greater increase.

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  2. What other viruses have been successfully introduced into cells via the use of ExpressMag beads?

    1 답변

    1. Adenovirus, SIV, Retrovirus, Measles virus and HIV have all been successfully introduced into cells using ExpressMag.

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  3. What is the Department of Transportation shipping information for this product?

    1 답변

    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

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  4. What cell lines have been transduced successfully using ExpressMag Beads?

    1 답변

    1. The following cell types have been successfully transduced using ExpressMag Beads: PBL, PBMC, T Cells, GMB primary tumor cells, A549, NYGM, T98G, U251, U87,YH-13, YK6-1, KS-1, NIH3T3, K562, Vero, HeLa, CHO, L929, B95a, H9, HUVEC, CD4+ Primary Human T-Cells, Human Bone Marrow Macrophages, Primary Keratinocytes, SKOV3, MM-AN, MEF and CD 34+.

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  5. How does the storage temperature relate to shipping conditions?

    1 답변

    1. The storage conditions that a Sigma-Aldrich catalog and label recommend for products are deliberately conservative. For many products, long-term storage at low temperatures will increase the time during which they are expected to remain in specification and therefore are labeled accordingly. Where short-term storage, shipping time frame, or exposure to conditions other than those recommended for long-term storage will not affect product quality, Sigma-Aldrich will ship at ambient temperature. The products sensitive to short-term exposure to conditions other than their recommended long-term storage are shipped on wet or dry ice. Ambient temperature shipping helps to control shipping costs for our customers. At any time, our customers can request wet- or dry-ice shipment, but the special handling is at customer expense if our product history indicates that the product is stable for regular shipment.

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  6. What MISSION products have been successfully introduced into cells using ExpressMag® beads?

    1 답변

    1. MISSION Lentiviral Particles (various targets/clones), SHC002V, SHC003V and SHC008V have been successfully introduced into cells using ExpressMag beads.

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