SHC012V

MISSION^® pLKO.1-puro-CMV-TagRFP^™ Positive Control Transduction Particles

Contains a gene encoding TagRFP

• 동의어(들): MISSION^®, MISSION^® Control Transduction Particles
• UNSPSC 코드: 41106609
• NACRES: NA.51

속성

• 제품 라인: MISSION^®
• 농도: ≥1x10⁶ VP/ml (via p24 assay)
• 기술: capture ELISA: 10⁶ TU/mL using p24
• 배송 상태: dry ice
• 저장 온도: −70°C

설명

일반 설명

Self-inactivating replication incompetent viral particles are produced in packaging cells (HEK293T) by co-transfection with compatible packaging plasmids. In addition, the Control Transduction Particles are pseudotyped with an envelope G glycoprotein from Vesicular Stomatitis Virus (VSV-G), allowing transduction of a wide variety of mammalian cells. 200 μl of 10⁶ TU/ml (via p24 titering assay) lentiviral particles are provided as frozen stock.

When conducting experiments using MISSION^® shRNA clones, the proper controls should be a key element of your experimental design to allow for accurate interpretation of knockdown results. The MISSION Control Transduction Particles are a critical positive control to monitor transduction efficiency.
To see more application data, protocols, vector maps visit sigma.com/shrna.

애플리케이션

MISSION^® pLKO.1-puro-CMV-TagRFP^™ Positive Control Transduction Particles has been used to knockdown transient receptor potential vanilloid 1 receptor (TRPV1)-like receptors. It has also been used to generate fluorescent cell lines.

MISSION^®pLKO.1-puro-CMV-TagRFP^™ Positive Control Transduction Particles has been used in generating fluorescent cell lines by transduction. It has also been used in transfection of human dermal fibroblast (HDF), WI-38 and IMR-90 FBs to express tagRFP.

To see more application data, protocols, vector maps visit sigma.com/shrna.

When conducting experiments using MISSION shRNA clones, the proper controls should be a key element of your experimental design to allow for accurate interpretation of knockdown results. The MISSION TagRFP Control Transduction Particles are a positive control to monitor transduction efficiency. The MISSION TagRFP Control Transduction Particles are produced from the sequence-verified 8594 base pair lentivirus plasmid, pLKO.1-puro-CMV-TagRFP (SHC012).

This construct aids in interpretation of experimental design and results by providing a red fluorescent protein marker for monitoring success in transduction of your cells of interest.

법적 정보

MISSION is a registered trademark of Merck KGaA, Darmstadt, Germany

TagRFP is a trademark of Evrogen Co.

안전성 정보

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 3
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable