SAE0111

Phosphatase, Alkaline from human placenta

≥10 units/mg solid (in glycine buffer)

• 동의어(들): Phosphatase, Alkaline from human placenta, Orthophosphoric-monoester phosphohydrolase (alkaline optimum)
• CAS Number: 9001-78-9
• 효소 위원회 번호: 3.1.3.1 (BRENDA, IUBMB)
• MDL number: MFCD00131849
• UNSPSC 코드: 12352200
• NACRES: NA.54

속성

• 생물학적 소스: human placenta
• 특이 활성도: ≥10 units/mg solid (in glycine buffer)
• UniProt 수납 번호: P05187
• 저장 온도: −20°C
• 유전자 정보: human ... ALPP(250)

설명

애플리케이션

Alkaline phosphatase is used for conjugation to antibodies and other proteins for ELISA, Western blotting, and histochemical detection. It is routinely used to dephosphorylate proteins and nucleic acids. It may be used for protein labeling when high sensitivity is required. Alkaline phosphatase may be also be used to dephosphorylate the 5′-termini of DNA or RNA to prevent self-ligation. DNA or RNA can also be tagged with radiolabeled phosphate (via T4 polynucleotide kinase) after dephosphorylation with alkaline phosphatase.

생화학적/생리학적 작용

Alkaline phosphatase dephosphorylates proteins and nucleic acids. Human placental alkaline phosphatase (PLAP) has an unique residue, Glu-429. It is an allosteric enzyme that is uncompetitively inhibited by L-Phe, L-Trp, and L-Leu. PLAP is post-translationally modified. Phosphatidylinositol-specific phospholipase C may be involved in the release of PLAP. PLAP may have a role in cell division and may be involved in the transfer of maternal IgG to the fetus.

단위 정의

One unit will hydrolyze 1.0 umole of P-Nitrophenyl Phosphate per minute at pH 10.4 at 37 Deg C.

안전성 정보

• 픽토그램: GHS08
• 신호어: Danger
• 유해 및 위험 성명서: H334
• 예방조치 성명서: P261 - P284 - P501
• Hazard Classifications: Resp. Sens. 1
• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 1
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable