SAB4200415

Anti-NEZHA antibody, Mouse monoclonal

clone NEZHA-1, purified from hybridoma cell culture

• 동의어(들): Anti-CAMSAP3, Anti-Calmodulin regulated spectrin-associated protein family member 3, Anti-KIAA, Monoclonal Anti-NEZHA antibody produced in mouse
• UNSPSC 코드: 12352203
• NACRES: NA.41

속성

• 생물학적 소스: mouse
• 결합: unconjugated
• 항체 형태: purified from hybridoma cell culture
• 항체 생산 유형: primary antibodies
• 클론: NEZHA-1, monoclonal
• 양식: buffered aqueous solution
• 분자량: antigen ~150 kDa
• 종 반응성: canine, human
• 농도: ~1.0 mg/mL
• 기술: immunoprecipitation (IP): suitable; indirect immunofluorescence: suitable; western blot: 2.5-5.0 μg/mL using whole extracts of human SW480 cells
• 동형: IgG1
• UniProt 수납 번호: Q9P1Y5
• 배송 상태: dry ice
• 저장 온도: −20°C
• 타겟 번역 후 변형: unmodified
• 유전자 정보: human ... CAMSAP3(57662) ; mouse ... Camsap3(69697)

설명

일반 설명

Monoclonal Anti-NEZHA (mouse IgG1 isotype) is derived from the hybridoma NEZHA-1 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a synthetic peptide corresponding to a sequence at the C-terminal region of human NEZHA, conjugated to KLH. Nezha (a deity in Chinese mythology) also known as KIAA1543 and calmodulin-regulated spectrin associated protein, is a 1276 amino acid protein that contains one calponin homology (CH) and two coiled coil (CC1 and 2) domains, as well as one DUF1781 (DUF) domain of unknown function. NEZHA is mapped to human chromosome 19p13.

NEZHA/ calmodulin regulated spectrin associated protein family member 3 (CAMSAP3) contains a conserved domain called cholecystokinin (CKK) domain at carboxy-terminal, as well as several coiled-coil regions and an calponin homology domain at amino-terminal.[1]

면역원

synthetic peptide corresponding to a sequence at the C-terminal region of human NEZHA, conjugated to KLH. The corresponding sequence is identical in mouse, rat, monkey and dog NEZHA.

애플리케이션

Monoclonal Anti-NEZHA antibody produced in mouse has been used in immunofluorescence staining.[1]

Monoclonal Anti-NEZHA antibody produced in mouse has been used in:

• immunofluorescence
• immunoprecipitation
• immunofluorescence
• mass-spectrometry-based analysis of a streptavidin pull-down assay
• immunostaining

생화학적/생리학적 작용

CAMSAP3 (calmodulin-regulated spectrin-associated protein 3)/ NEZHA modulates the minus-end dynamics of microtubules. It helps to maintain neuronal polarity. Absence of this gene results in the formation of supernumerary axon.

NEZHA/ calmodulin regulated spectrin associated protein family member 3 (CAMSAP3) facilitates translocation of Golgi vesicles in epithelial cells. In addition, it also helps in positioning the apical-to-basal polarity of microtubules in epithelial cells.

물리적 형태

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

안전성 정보

• Storage Class Code: 10 - Combustible liquids
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable