SAB4200232

Anti-GAD65 antibody , Mouse monoclonal

clone N-GAD65, purified from hybridoma cell culture

• 동의어(들): Anti-GAD2, Anti-glutamate decarboxylase 2 (pancreatic islets and brain, 65kDa), Anti-glutamate decarboxylase 65 kDa isoform
• MDL number: MFCD03455043
• UNSPSC 코드: 12352203
• NACRES: NA.41

속성

• 생물학적 소스: mouse
• 결합: unconjugated
• 항체 형태: purified from hybridoma cell culture
• 항체 생산 유형: primary antibodies
• 클론: N-GAD65, monoclonal
• 형태: buffered aqueous solution
• 분자량: antigen ~65 kDa
• 종 반응성: human, mouse, monkey, rat
• 농도: ~1.0 mg/mL
• 기술: immunohistochemistry: suitable; immunoprecipitation (IP): suitable; western blot: 0.25-0.5 μg/mL using whole extracts of rat brain
• 동형: IgG1
• UniProt 수납 번호: Q05329
• 배송 상태: dry ice
• 저장 온도: −20°C
• 타겟 번역 후 변형: unmodified
• 유전자 정보: human ... GAD2(2572) ; mouse ... Gad2(14417) ; rat ... Gad2(24380)

설명

일반 설명

Glutamate decarboxylase 2 (GAD2) encodes GAD65, an isoform of glutamate decarboxylase. GAD65 is majorly expressed in axon terminal regions in neurons. GAD65 is present in the synaptic vesicles. GAD2 is mapped to human chromosome 10p12.1. It has an N-terminal pyridoxal 5′-phosphate (PLP) binding −domain.

애플리케이션

Monoclonal Anti-GAD65 antibody produced in mouse has been used in the detection of recombinant GAD65 using ELISA.

생화학적/생리학적 작용

Glutamate decarboxylase 2 (GAD2) protein GAD65 catalyzes the formation of γ-aminobutyric acid (GABA) from glutamic acid. Palmitoylation of GAD65 is crucial for protein sorting in synaptic clusters. GAD65 autoantigen is prevalent in type 1 diabetes. Its expression increases on allergen exposure. GAD65, along with γ-aminobutyric acid (GABA), may play a key role in the pathogenesis of asthma.

물리적 형태

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

안전성 정보

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable