SAB4200218

Anti-Glycogen Synthase 1 (N-terminal) antibody produced in rabbit

~1.5 mg/mL, affinity isolated antibody

• 동의어(들): Anti-GYS1, Anti-Glycogen synthase 1 (muscle)
• UNSPSC 코드: 12352203
• NACRES: NA.41

속성

• 생물학적 소스: rabbit
• 결합: unconjugated
• 항체 형태: affinity isolated antibody
• 항체 생산 유형: primary antibodies
• 클론: polyclonal
• 양식: buffered aqueous solution
• 분자량: antigen ~84 kDa
• 종 반응성: mouse, rat
• 농도: ~1.5 mg/mL
• 기술: immunoprecipitation (IP): 5-10 μg using rat brain extracts (S1 fraction); indirect immunofluorescence: 5-10 μg/mL using NIH3T3 cells; western blot: 1.5-3 μg/mL using mouse brain extracts (S1 fraction)
• UniProt 수납 번호: Q9Z1E4
• 배송 상태: dry ice
• 저장 온도: −20°C
• 타겟 번역 후 변형: unmodified
• 유전자 정보: human ... GYS1(2997)

설명

일반 설명

The glycogen synthase 1 (GYS1) gene is mapped to human chromosome 19q13.3. In mammals, two glycogen synthase (GS) isoforms have been identified, the muscle GS form, glycogen synthase 1 (GYS1) and the liver specific form, glycogen synthase 2 (GYS2, LGS). GYS1 is predominantly expressed in the skeletal and cardiac muscles.

특이성

Anti-Glycogen Synthase 1 (N-terminal), specifically recognizes mouse and rat glycogen synthase 1.

애플리케이션

Anti-Glycogen Synthase 1 (N-terminal) antibody produced in rabbit is suitable for:

• immunoblotting
• immunoprecipitation
• immunofluorescence

생화학적/생리학적 작용

Glycogen synthase (GS or GYS), the rate-limiting enzyme for glycogen biosynthesis, catalyzes the incorporation of α-1,4-linked glucose units into glycogen chains. Both, the muscle (GYS1) and the liver GS (GYS2) forms are highly regulated by phosphorylation, glucose availability, glycogen levels and allosteric effectors. In response to hormonal stimuli, GS is phosphorylated on up to nine serine residues resulting in progressive inactivation of the two isoforms. GS activity is stimulated by insulin in liver, muscle and adipose tissues. The enzyme undergoes dephosphorylation allosteric activation and thus translocates between various subcellular compartments. Insulin increases GS activity primarily by dephosphorylation of four key residues, a process thought to be mediated by the activation of protein phosphatase-1 (PP1) and the inactivation of glycogen synthase kinase-3 (GSK3). GYS1 deficiency might lead to congenital disorders associated with glycogen metabolism.

물리적 형태

Solution in 0.01 M phos­phate buffered saline, pH 7.4, containing 15 mM sodium azide.

저장 및 안정성

For continuous use, store at 2–8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

안전성 정보

• Storage Class Code: 10 - Combustible liquids
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable