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Merck
모든 사진(1)

문서

S6402

Sigma-Aldrich

Streptavidin−Cy3 from Streptomyces avidinii

buffered aqueous solution

동의어(들):

Streptavidin Cy3

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About This Item

MDL number:
UNSPSC 코드:
12352203
NACRES:
NA.46

결합

CY3 conjugate

형태

buffered aqueous solution

라벨링 범위

3-9 mol fluorochrome per mol protein

기술

immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:100

배송 상태

wet ice

저장 온도

2-8°C

일반 설명

Streptavidin is a homotetramer protein. One molecule of streptavidin binds four molecules of biotin by a non-covalent interaction that is essentially irreversible. Streptavidin has a high affinity for biotin and is extensively used as a labeling tool and for studies involving binding proteins.

애플리케이션

Streptavidin−Cy3 from Streptomyces avidinii has been used:
  • in double immunofluorescence staining to detect biotinylated lectin
  • for insulin and C-peptide staining in immunofluorescence analysis
  • in double immunofluorescence to verify the presence of cells filled with biocytin
  • in conjunction with biotinylated antibodies in immunohistochemistry and immunocytochemistry
  • with the traditional rhodamine filter arrangements due to the spectral overlap

특징 및 장점

Spectral Characteristics of Cy3 Dye:
  • Absorbance Max 552 nm
  • Emission Max 565 nm
  • Molar Extinction Coefficient 1.3 x 10-5 M-1cm-1

물리적 형태

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin, and 15 mM sodium azide

법적 정보

Cy3 is a trademark of Cytiva

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

개인 보호 장비

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)


시험 성적서(COA)

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문서 라이브러리 방문

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We established a preadipocyte cell line from mature adipocytes obtained from subcutaneous fat tissue of green fluorescent protein (GFP) transgenic mice. The floating top layer, containing mature adipocytes, was isolated from subcutaneous fat tissue by collagenase digestion and filtration. Fluorescence-activated
Agnieszka E Gorska et al.
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We have previously shown that expression of a dominant-negative type II transforming growth factor-beta receptor (DNIIR) in mammary epithelium under control of the MMTV promoter/enhancer causes alveolar hyperplasia and differentiation in virgin mice. Here we show that MMTV-DNIIR female mice
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eLife, 8 (2019-09-10)
Most of our knowledge on human CNS circuitry and related disorders originates from model organisms. How well such data translate to the human CNS remains largely to be determined. Human brain slice cultures derived from neurosurgical resections may offer novel
Ji-Myung Bae et al.
Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research, 33(6), 1126-1140 (2018-02-07)
The Sp7/Osterix transcription factor is essential for bone development. Mutations of the Sp7 gene in humans are associated with craniofacial anomalies and osteogenesis imperfecta. However, the role of Sp7 in embryonic tooth development remains unknown. Here we identified the functional
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Nature immunology, 19(6), 606-616 (2018-05-20)
Although much is known about the physiological framework of T cell motility, and numerous rate-limiting molecules have been identified through loss-of-function approaches, an integrated functional concept of T cell motility is lacking. Here, we used in vivo precision morphometry together

문서

사용 가능한 형광 동소 교잡법(FISH) 절차, 시약, 장비.

Available Fluorescent in situ hybridization (FISH) procedures, reagents and equipment.

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