S5174

SPARC from murine parietal yolk sac (PYS-2) cells

~80% (SDS-PAGE), lyophilized powder

• 동의어(들): BM-40, Osteonectin, Secreted protein acidic and rich in cysteine
• MDL number: MFCD00213018
• UNSPSC 코드: 12352200
• NACRES: NA.32

속성

• 생물학적 소스: mouse (PYS-2 cells)
• 분석: ~80% (SDS-PAGE)
• 양식: lyophilized powder
• 기술: cell culture | mammalian: suitable
• 표면 범위: 4‑40 μg/cm²
• UniProt 수납 번호: P07214
• 배송 상태: dry ice
• 저장 온도: −20°C
• 유전자 정보: mouse ... Sparc(20692)

설명

애플리케이션

SPARC has many useful properties including inhibiting cell spreading and diminishing focal contacts in vitro, abrogating the effects of PDGF and beta-FGF, binding directly to several components of the ECM, and undergoes proteolysis that exposes and releases regions in the protein that correlates with biological activities.

생화학적/생리학적 작용

SPARC is expressed in many tissues including bone, skin, skeletal muscle, reproductive tissues, and embryonic connective tissue. It is expressed in high levels in tissues that are undergoing morphogenesis, remodeling and repair.

성분

SPARC, or Secreted Protein Acidic and Rich in Cysteine, is a 283-287 AA Ca²⁺ binding to glycoprotein with an apparent molecular weight of 40-43 kDa.

주의사항

The lyophilized powder should be stored frozen at -20°C. The reconstituted product should be stored frozen at -70°C.

제조 메모

This product is supplied as a lyophilized powder from phosphate buffered saline at 25 μg protein/vial. It should be reconstituted aseptically with sterile water, producing a solution of 0.1 to 0.3 mg protein per mL. SPARC is initially purified from mouse parietal yolk sac cells under conditions that preserve biological activity.

안전성 정보

• Storage Class Code: 13 - Non Combustible Solids
• WGK: WGK 3
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable
• 개인 보호 장비: Eyeshields, Gloves, type N95 (US)