S3917

Single-strand Binding Protein from Escherichia coli

>95% (SDS-PAGE), for molecular biology, Single strand binding protein to enhance PCR specificity

• 동의어(들): SSB
• MDL number: MFCD00213047
• UNSPSC 코드: 12352202
• NACRES: NA.52

속성

• Grade: Molecular Biology; for molecular biology
• Quality Level: 200
• 분석: >95% (SDS-PAGE)
• 양식: liquid
• 색상: colorless
• 외래 활성: DNase and RNase, none detected
• 배송 상태: wet ice
• 저장 온도: −20°C

설명

일반 설명

Single-strand Binding Protein is a homotetramer,[1] found in E. coli during the event of DNA metabolism.[2]

애플리케이션

Binds with high specificity to single-stranded DNA. Can be useful in enhancing the specificity of PCR and in enabling the sequencing of problematic DNA templates.

Single-strand Binding Protein from Escherichia coli has been used in in vitro RNA/Single-Strand Binding Protein (SSB) complex formation assays[3] and in protein purification to determine integrity and functionality of the integron attC sites.[4]

생화학적/생리학적 작용

Single-strand Binding Protein (SSBP) exhibits high affinity towards single stranded DNA important for DNA metabolic processes.[1][5] Single-stranded DNA-binding protein hSSB1 is critical for genomic stability and assists the delivery of primers during the formation of lagging standard.[5] It also prevents the formation of inhibitory secondary structures in the exposed lagging-strand of DNA during replication.[5] SSBP mediates the recruitment of other proteins to their sites of function on DNA.[1]

물리적 형태

Solution in 20 mM Tris-HCl, pH 8.0, 0.5 M NaCl, 0.1 mM EDTA, 0.1 mM DTT, 50% glycerol

안전성 정보

• 픽토그램: GHS05,GHS09
• 신호어: Danger
• 유해 및 위험 성명서: H318,H410
• 예방조치 성명서: P273 - P280 - P305 + P351 + P338 - P391 - P501
• Hazard Classifications: Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1
• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 2