RDD002

HEPES

≥99.5%, crystalline powder, anhydrous, Redi-Dri^™

• 동의어(들): 4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid, N-(2-Hydroxyethyl)piperazine-N′-(2-ethanesulfonic acid)
• 실험식(Hill 표기법): C₈H₁₈N₂O₄S
• CAS Number: 7365-45-9
• Molecular Weight: 238.30
• Beilstein: 883043
• EC Number: 230-907-9
• MDL number: MFCD00006158
• UNSPSC 코드: 12161700
• PubChem Substance ID: 329824215
• NACRES: NA.25

속성

• 제품명: HEPES, anhydrous, free-flowing, Redi-Dri^™, ≥99.5%
• Grade: anhydrous
• Quality Level: 100
• 제품 라인: Redi-Dri^™
• 분석: ≥99.5%
• 양식: crystalline powder
• 품질: free-flowing
• pH: 5.0-6.5 (25 °C, 238 g/L)
• 유용한 pH 범위: 6.8-8.2
• pKa(25 °C): 7.5
• solubility: water: 500 mg/mL, clear, colorless
• SMILES string: OCCN1CCN(CC1)CCS(O)(=O)=O
• InChI: 1S/C8H18N2O4S/c11-7-5-9-1-3-10(4-2-9)6-8-15(12,13)14/h11H,1-8H2,(H,12,13,14)
• InChI key: JKMHFZQWWAIEOD-UHFFFAOYSA-N

설명

일반 설명

HEPES has been described as one of the best all-purpose buffers available for biological research.[1] At biological pH, the molecule is zwitterionic, and is effective as a buffer at pH 6.8 to 8.2. HEPES has been used in a wide variety of applications, including tissue culture. It is commonly used to buffer cell culture media in air. HEPES finds its usage in in vitro experiments on Mg.[2]

애플리케이션

HEPES has been used:

• In the novel vesicle-budding protocol as a component in stock solution and buffer solutions[3]
• As a component in intracellular solution for the preparation of brain-slice recording[4]
• As a component of wash buffer, elution buffer and desalting buffer during affinity protein purification[5]
• To maintain the pH at 7 in thermal denaturation assay of purified protein[5]
• In isocratic elution of protein using gel filtration[5]
• Along with NaCl in sample preparation for EPR (Electron Paramagnetic Resonance) spectroscopy[5]

법적 정보

Redi-Dri is a trademark of Sigma-Aldrich Co. LLC

안전성 정보

• Storage Class Code: 13 - Non Combustible Solids
• WGK: WGK 1
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable