R1656

RAF1 (EE) (306-end), active, GST tagged human

PRECISIO^® Kinase, recombinant, expressed in baculovirus infected Sf9 cells, ≥70% (SDS-PAGE), buffered aqueous glycerol solution

• 동의어(들): CMD1NN, NS5, RAF proto-oncogene serine/threonine-protein kinase, cRaf
• UNSPSC 코드: 12352200
• NACRES: NA.32

속성

• 생물학적 소스: fermentation/recombinant
• Quality Level: 200
• 재조합: expressed in baculovirus infected Sf9 cells
• 제품 라인: PRECISIO^® Kinase
• 분석: ≥70% (SDS-PAGE)
• 양식: buffered aqueous glycerol solution
• 특이 활성도: 5100-6900 nmol/min·mg
• 분자량: ~63 kDa
• 제조업체/상표: Precision
• 기술: cell based assay: suitable
• solubility: water: soluble
• 단백질 ID 수납 번호: NM 002880
• UniProt 수납 번호: P04049
• 배송 상태: dry ice
• 저장 온도: −70°C
• 유전자 정보: human ... RAF1(5894)

설명

일반 설명

Research area: Cell Signaling

Rapidly Accelerated Fibrosarcoma (RAF) belongs to receptor tyrosine kinase (RTK) family. RAF exists as three isoforms A, B and C-RAF or RAF-1. [1] RAF1 links Ras family members with mitogen-activated protein kinase/extracellular signal-regulated kinase (MAPK/ERK) signaling cascade.[2] The RAF-1 gene is mapped to the human chromosome at 3p25.2.[3]

생화학적/생리학적 작용

Rapidly Accelerated Fibrosarcoma 1(RAF1), a member of MAP kinase kinase kinase (MAP3K) family plays a vital role in cell signaling by directly interacting with GTPases and functions downstream in their signaling pathway.[4] RaF1 is activated by Ras in the presence of growth-promoting signals, leading to the activation of mitogen-activated protein kinase kinase (MEK1/MEK2) and extracellular signal-regulated protein kinases 1 and 2 (ERK1/ERK2) downstream. [5] The activated ERK proteins control gene expression related to cell division, apoptosis, cell differentiation, and cell migration.[4] The gene is mutated or upregulated in prostate and bladder cancer.[5] Also, mutations are associated with Noonan syndrome, which leads to various physical abnormalities like craniofacial dysmorphisms, ocular, and musculoskeletal irregularities, neurocognitive impairment, and cardiac malformations.[4]

물리적 형태

Supplied in 50 mM Tris-HCl, pH 7.5, with 150 mM NaCl, 0.2 5mM DTT, 0.1 mM EGTA, 0.1 mM EDTA, 0.1 mM PMSF, and 25% glycerol.

법적 정보

PRECISIO is a registered trademark of Merck KGaA, Darmstadt, Germany

안전성 정보

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 1
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable
• 개인 보호 장비: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)