추천 제품
사용
kit sufficient for 25 mini-gels (10 × 10 cm)
Quality Level
기술
protein staining: suitable
저장 온도
20-25°C
일반 설명
Conveniently packaged, extremely stable, and highly sensitive, ProteoSilver™ Plus Silver Stain Kit is an ideal product for any protein scientist. Similar to ProteoSilver (PROTSIL1), this kit contains prepared solutions of silver staining reagents along with detailed instructions to achieve optimal results. With a detection limit of 0.1 ng/mm2 of protein (BSA) and an extremely low background, ProteoSilver Plus leads to superior detection of low abundance proteins.
ProteoSilver Plus is also MALDI-MS compatible and does not contain glutaraldehyde, which crosslinks lysine residues, resulting in inaccurate spectra. ProteoSilver Plus Silver Stain Kit contains two additional reagents for destaining an excised protein spot, for those wishing to perform further characterization through tryptic digestion and MALDI-MS analysis.
ProteoSilver Plus is also MALDI-MS compatible and does not contain glutaraldehyde, which crosslinks lysine residues, resulting in inaccurate spectra. ProteoSilver Plus Silver Stain Kit contains two additional reagents for destaining an excised protein spot, for those wishing to perform further characterization through tryptic digestion and MALDI-MS analysis.
애플리케이션
ProteoSilver Silver Stain Kit has been used for staining of the gels for visualizing proteins. It has also been used for destaining of the protein of interest using destainer provided in the kit for mass spectroscopic analysis.
특징 및 장점
- Premixed and preweighed solutions reduce time and cost of purchasing and preparing individual components
- Optimized protocols help establish conditions for best results
- High sensitivity and low background ensure very low abundance proteins can be detected and resolved from other proteins
- MALDI Compatible - Protein spots of interest can be further characterized by mass spectrometry
- Room Temperature Stability allows easy and convenient storage
법적 정보
ProteoSilver is a trademark of Sigma-Aldrich Co. LLC
신호어
Danger
Hazard Classifications
Acute Tox. 2 Inhalation - Acute Tox. 3 Dermal - Acute Tox. 3 Oral - Aquatic Acute 1 - Aquatic Chronic 1 - Carc. 1B - Eye Dam. 1 - Met. Corr. 1 - Muta. 2 - Repr. 1B - Skin Corr. 1A - Skin Sens. 1 - STOT SE 2 - STOT SE 3
표적 기관
Eyes,Central nervous system, Respiratory system
Storage Class Code
6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials
WGK
WGK 3
Flash Point (°F)
149.0 °F - closed cup
Flash Point (°C)
65 °C - closed cup
가장 최신 버전 중 하나를 선택하세요:
시험 성적서(COA)
Effect of biomolecules from human renal matrix of calcium oxalate monohydrate (CaOx) stones on in vitro calcium phosphate crystallization.
International Brazilian Journal of Urology : Official Journal of the Brazilian Society of Urology, 36, 621-628 (2010)
2D map of proteins from human renal stone matrix and evaluation of their effect on oxalate induced renal tubular epithelial cell injury.
International Brazilian Journal of Urology : Official Journal of the Brazilian Society of Urology, 39, 128-136 (2013)
International braz j urol : official journal of the Brazilian Society of Urology, 36(5), 621-628 (2010-11-04)
Investigate the activity of high and low molecular weight biomolecules present in the matrix of human calcium oxalate (CaOx) stones not only on the initial mineral phase formation of calcium and phosphate (CaP) but also on its growth and demineralization
PloS one, 8(7), e69916-e69916 (2013-07-31)
The increasing number of patients suffering from urolithiasis represents one of the major challenges which nephrologists face worldwide today. For enhancing therapeutic outcomes of this disease, the pathogenic basis for the formation of renal stones is the need of hour.
Molecular & cellular proteomics : MCP, 6(2), 193-206 (2006-11-10)
The study of protein interactions constitutes an important domain to understand the physiology and pathogenesis of microorganisms. The two-dimensional blue native/SDS-PAGE was initially reported to analyze membrane protein complexes. In this study, both cytoplasmic and membrane complexes of a bacterium
문서
Sigma offers EZBlue™ and ProteoSilver™ reagents for protein visualization, suitable for proteomics and traditional PAGE formats.
자사의 과학자팀은 생명 과학, 재료 과학, 화학 합성, 크로마토그래피, 분석 및 기타 많은 영역을 포함한 모든 과학 분야에 경험이 있습니다..
고객지원팀으로 연락바랍니다.