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Merck
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G1041

Sigma-Aldrich

EZBlue™ Gel Staining Reagent

동의어(들):

protein gel stain, protein stain

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About This Item

UNSPSC 코드:
41105300
NACRES:
NA.32

기술

protein staining: suitable

Quality Level

200

저장 온도

2-8°C

일반 설명

Convenient, sensitive, and safe, EZBlue™ Coomassie Brilliant Blue G-250 colloidal protein stain improves protein electrophoresis results while significantly reducing staining time. Conveniently packaged, EZBlue requires no messy weigh-ups or additions of methanol or acid. As a colloidal stain, it reacts only with proteins, not the gel itself. Background staining is reduced, so protein bands can be visualized almost immediately. No destaining step is required, although a water wash may intensify bands and clarify the background. Most impressively, EZBlue is extremely sensitive, detecting as little as 5 ng of protein.

애플리케이션

EZBlue Gel Staining Reagent has been used as a staining reagent in sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE).

특징 및 장점

  • Premixed solution eliminates the time and effort required to prepare the stain
  • Increased sensitivity ensures that low abundance proteins can be detected (as little as 5 ng)
  • Rapid reaction significantly reduces the amount of time required to stain and rinse
  • No solvent waste so you save time and money by eliminating hazardous material disposal

기타 정보

A ready-to-use Brilliant Blue G-250 based protein stain for "one step" ultrasensitive detection on polyacrylamide gels and PVDF membranes.

법적 정보

EZBlue is a trademark of Sigma-Aldrich Co. LLC

픽토그램

Health hazardCorrosion
GHS08,GHS05

신호어

Warning

유해 및 위험 성명서

H290,H315,H319,H371

Hazard Classifications

Eye Irrit. 2 - Met. Corr. 1 - Skin Irrit. 2 - STOT SE 2

표적 기관

Eyes,Central nervous system

Storage Class Code

8A - Combustible corrosive hazardous materials

WGK

WGK 2

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

개인 보호 장비

Faceshields, Gloves, Goggles, type ABEK (EN14387) respirator filter

가장 최신 버전 중 하나를 선택하세요:

시험 성적서(COA)

Lot/Batch Number
SLCN0167
SLCC3649
SLBZ0323
SLBX5633
SLBW2004

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COA 검색

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문서

Cell Lysate Preparation

Cell lysis challenges and protein extraction variables for Western blot sensitivity and reproducibility.

Western Blot Analysis of Immunoprecipitation (IP-Western)

The possible causes and potential remedies for challenges encountered in the immunoprecipitation-Western blot technique, which consists of cell lysis, formation of the antibody-antigen (immune) complex, precipitation of the immune complexes, and analysis by Western blotting.

Rapid Glycan Prep & Analysis by LC-MS Workflow

PNGase Fast denaturing buffer and enzyme provide results similar to a conventional 20-hour protocol, reducing workflow time to about 1 hour.

Protein Staining Reagents Selection Guide

Sigma offers EZBlue™ and ProteoSilver™ reagents for protein visualization, suitable for proteomics and traditional PAGE formats.

모두 보기

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mPAGE® TurboMix Bis-Tris 겔 캐스팅 키트를 이용한 PAGE/SDS-PAGE용 핸드캐스팅 겔

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Simplify your SDS-PAGE gel preparation with our pre-mixed solutions and protocols to achieve precise protein separation.

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