Quality Segment
usage
kit sufficient for ≤100 applications
technique(s)
protein mass spectrometry: suitable
storage temp.
2-8°C
General description
The Trypsin Profile IGD Kit enables fast, efficient, and complete in-gel tryptic digestion of up to 100 excised protein spots. Digested proteins are ready for MALDI-MS (matrix-assisted laser desorption/ionization-mass spectrometry) and require no additional preparation. Because the Trypsin Profile IGD Kit contains Proteomics Grade Trypsin, a higher sequence coverage and fewer ambiguous autolytic peaks are observed in MALDI spectra.
Trypsin has been chemically modified through reductive methylation of the ε-amino groups of lysine to reduce autolysis and minimize autolytic fragments. In addition, it has been TPCK (tosyl phenylalanyl chloromethyl ketone) treated to remove residual chymotrypsin activity and then further purified by affinity chromatography, yielding a highly purified trypsin suitable for proteomics work.
Trypsin has been chemically modified through reductive methylation of the ε-amino groups of lysine to reduce autolysis and minimize autolytic fragments. In addition, it has been TPCK (tosyl phenylalanyl chloromethyl ketone) treated to remove residual chymotrypsin activity and then further purified by affinity chromatography, yielding a highly purified trypsin suitable for proteomics work.
Application
Features and Benefits
- Faster destaining than with alternative in-gel digest kits
- Includes 100% of the reagents needed to destain, digest, and extract proteins/peptides of interest
- Compatible with Coomassie, SYPRO™ Orange, SYPRO Ruby, and ProteoSilver™ Stained gels
- Resulting samples are ready for analysis by MALDI-MS or HPLC-MS
Legal Information
ProteoSilver is a trademark of Sigma-Aldrich Co. LLC
SYPRO is a trademark of Thermo-Fisher Scientific, Inc.
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이 품목 | |||
|---|---|---|---|
| description - | description - | description recombinant, expressed in Pichia pastoris, Proteomics Grade, liquid | description Proteomics Grade, lyophilized powder, recombinant, expressed in Pichia pastoris |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
| usage kit sufficient for ≤100 applications | usage - | usage - | usage - |
| storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C |
키트 구성품 전용
제품 번호
설명
- Destaining Solution Reconstituted to 75
경고어
Danger
Hazard Classifications
Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Eye Irrit. 2 - Flam. Liq. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
표적 장기
Respiratory system
저장 등급
3 - Flammable liquids
인화점 (F)
35.6 °F - closed cup
인화점 (°C)
2.0 °C - closed cup
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