P7623

PKD2 Active human

recombinant, expressed in E. coli, ≥70% (SDS-PAGE), aqueous glycerol solution

• 동의어(들): Protein Kinase D2
• MDL number: MFCD07371065
• UNSPSC 코드: 12352200
• NACRES: NA.32

속성

• 재조합: expressed in E. coli
• Quality Level: 200
• 분석: ≥70% (SDS-PAGE)
• 양식: aqueous glycerol solution
• 특이 활성도: 100 units/mg protein (Bradford)
• 분자량: protein apparent mol wt ~140 kDa
• UniProt 수납 번호: Q9BZL6
• 배송 상태: dry ice
• 저장 온도: −70°C
• 유전자 정보: human ... PRKD2(25865)

설명

애플리케이션

Kinase activity is measured as the molar amount of phosphate incorporated into the CREBTIDE substrate peptide (KRREILSRRPSYR), per minute per mg protein at 30 °C using a final concentration of 50 μM [³²P] ATP.

생화학적/생리학적 작용

PKD2 is a novel phorbol ester- and growth factor-stimulated serine/threonine kinase that contains two cysteine-rich motifs at the N terminus, a pleckstrin homology domain, and a catalytic domain. The PKD family of enzymes have been implicated in very diverse cellular functions, including Golgi organization and plasma membrane directed transport, metastasis, immune responses, apoptosis and cell proliferation.

단위 정의

One unit will incorporate one nanomole of phosphate into the CREBTIDE substrate (KRREILSRRPSYR) per minute at 30 °C at pH 7.2 using a final concentration of 50 μM [32P] ATP.

물리적 형태

Solution of 50 μl in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.25 mM dithiothreitol (DTT), 0.1 mM EGTA, and 30% glycerol.

안전성 정보

• Storage Class Code: 10 - Combustible liquids