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Merck
모든 사진(2)

주요 문서

P4600

Sigma-Aldrich

ReadyMix Taq PCR Reaction Mix

with MgCl2

동의어(들):

PCR master mix, Taq PCR master mix

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About This Item

MDL number:
UNSPSC 코드:
41106300
NACRES:
NA.55

Quality Level

양식

liquid

사용

sufficient for 100 reactions

특징

dNTPs included
hotstart: no

농도

1.5 units/reaction (50 μL reaction volume)

기술

PCR: suitable

색상

colorless

입력

purified DNA

응용 분야

agriculture

배송 상태

wet ice

저장 온도

−20°C

유사한 제품을 찾으십니까? 방문 제품 비교 안내

일반 설명

ReadyMix Taq PCR Reaction Mix is a prepared solution containing everything needed for a PCR reaction except the specific primers and template. The mix includes Sigma′s high quality Taq DNA Polymerase, 99% pure deoxynucleotides and buffer in a 2× optimized reaction concentrate. For reaction set-up, add the ReadyMix (25 μL) to the primers, template and water (total volume 50 μL). Using ReadyMix Taq PCR Reaction Mix reduces pipetting steps and risk of contamination. This saves time and reduces errors while still providing the great performance of Sigma′s Taq DNA Polymerase.

애플리케이션

ReadyMix Taq PCR Reaction Mix has been used:
  • in the development and specificity test of the amplified fragment length polymorphism (AFLP) markers
  • in the PCR reaction for terminal restriction fragment length polymorphism (T-RFLP) analysis
  • in the molecular identification of yeasts
  • for routine PCR amplifications

특징 및 장점

  • Amplifies targets up to 7 kb in length

  • ReadyMix Taq PCR Reaction Mix offers the same great performance as Taq DNA Polymerase in a more convenient formulation

  • ReadyMix format reduces setup time.

  • Reduced risk of contamination from multiple pipet steps.

포장

Default reaction volume is 50 μL

100RXN is packaged as 1 X 2.5 mL
Supplied with a vial of PCR grade water for dilution.

단위 정의

One unit incorporates 10 nmol of total dNTPs into acid-precipitable DNA in 30 min. at 74 °C.

법적 정보

Use of this product is covered by one or more of the following US patents and corresponding patent claims outside the US: 5,789,224, 5,618,711, 6,127,155 and claims outside the US corresponding to expired US Patent No. 5,079,352. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser′s own internal research. No right under any other patent claim, no right to perform any patented method, and no right to perform commercial services of any kind, including without limitation reporting the results of purchaser′s activities for a fee or other commercial consideration, is conveyed expressly, by implication, or by estoppel. This product is for research use only. Diagnostic uses under Roche patents require a separate license from Roche. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.
ReadyMix is a trademark of Sigma-Aldrich Co. LLC

관련 제품

제품 번호
설명
가격

픽토그램

Exclamation markEnvironment

신호어

Warning

유해 및 위험 성명서

Hazard Classifications

Aquatic Acute 1 - Aquatic Chronic 1 - Eye Irrit. 2 - Skin Irrit. 2 - Skin Sens. 1

Storage Class Code

10 - Combustible liquids

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable


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시험 성적서(COA)

Lot/Batch Number

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문서 라이브러리에서 최근에 구매한 제품에 대한 문서를 찾아보세요.

문서 라이브러리 방문

Assessment of fecal microflora changes in pigs supplemented with herbal residue and prebiotic
Samanta A K, et al.
PLoS ONE, 10(7), e0132961-e0132961 (2015)
Development of amplified fragment length polymorphism-derived functional strain-specific markers to assess the persistence of 10 bacterial strains in soil microcosms
Xiang S R, et al.
Applied and Environmental Microbiology, 76(21), 7126-7135 (2010)
Chitosan inactivates spoilage yeasts but enhances survival of Escherichia coli O157: H7 in apple juice
Kisko G, et al.
Journal of Applied Microbiology, 98(4), 872-880 (2005)
Ashis Kumar Samanta et al.
PloS one, 10(7), e0132961-e0132961 (2015-07-16)
Antibiotic usage in animals as a growth promoter is considered as public health issue due to its negative impact on consumer health and environment. The present study aimed to evaluate effectiveness of herbal residue (ginger, Zingiber officinale, dried rhizome powder)
A Dimitroglou et al.
Journal of animal science, 87(10), 3226-3234 (2009-07-21)
A study was conducted to investigate the effect of mannan oligosaccharide (MOS) on the gut microbiota and intestinal morphology of rainbow trout under commercial farming conditions. Juvenile (mean initial BW 38.2 +/- 1.7 g) and subadult (111.7 +/- 11.6 g)

문서

Explore PCR's history, from discovery to Nobel Prize. Discover real-time PCR (qPCR) and digital PCR developments.

PCR master mix simplifies PCR/RT-PCR with components like DNA polymerase, dNTPs, MgCl2, and buffer, available commercially or DIY.

Polymerase chain reaction (PCR) is a technique that results in exponential amplification of a target DNA sequence.

프로토콜

Learn standard PCR protocol steps and review reagent lists or cycling parameters. This method for routine PCR amplification of DNA uses standard Taq DNA polymerase.

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